Gene description for Prkar1a
Gene name protein kinase, cAMP dependent regulatory, type I, alpha
Gene symbol Prkar1a
Other names/aliases 1300018C22Rik
RIalpha
Tse-1
Tse1
Species Mus musculus
 Database cross references - Prkar1a
ExoCarta ExoCarta_19084
Vesiclepedia VP_19084
Entrez Gene 19084
UniProt Q9DBC7  
 Prkar1a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkar1a
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IBA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IMP
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 IGI
    cAMP-dependent protein kinase regulator activity GO:0008603 IMP
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    cAMP binding GO:0030552 IBA
    cAMP binding GO:0030552 IEA
    cAMP binding GO:0030552 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A catalytic subunit binding GO:0034236 IBA
    protein kinase A catalytic subunit binding GO:0034236 IPI
    protein kinase A catalytic subunit binding GO:0034236 ISO
Biological Process
    mesoderm formation GO:0001707 IMP
    protein phosphorylation GO:0006468 TAS
    negative regulation of protein kinase activity GO:0006469 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    heart development GO:0007507 IMP
    animal organ morphogenesis GO:0009887 TAS
    mesenchymal cell proliferation GO:0010463 TAS
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of insulin secretion GO:0032024 IMP
    sarcomere organization GO:0045214 IMP
    negative regulation of activated T cell proliferation GO:0046007 IEA
    negative regulation of activated T cell proliferation GO:0046007 ISO
    cardiac muscle cell proliferation GO:0060038 IMP
    cellular response to glucagon stimulus GO:0071377 IMP
    negative regulation of cAMP/PKA signal transduction GO:0141162 IEA
    negative regulation of cAMP/PKA signal transduction GO:0141162 ISO
Subcellular Localization
    immunological synapse GO:0001772 IEA
    immunological synapse GO:0001772 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 TAS
    multivesicular body GO:0005771 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    nucleotide-activated protein kinase complex GO:0031588 IEA
    nucleotide-activated protein kinase complex GO:0031588 ISO
    neuromuscular junction GO:0031594 IDA
    protein-containing complex GO:0032991 ISO
    plasma membrane raft GO:0044853 IEA
    plasma membrane raft GO:0044853 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    sperm head-tail coupling apparatus GO:0120212 IDA
 Experiment description of studies that identified Prkar1a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkar1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lnx1  
Affinity Capture-MS Mus musculus
2 Hcn1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Fancd2  
Affinity Capture-MS Mus musculus
6 Lnx2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Prkar1a is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Aquaporin-mediated transport IEA Reactome
Ca-dependent events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
DAG and IP3 signaling IEA Reactome
DARPP-32 events IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G-protein mediated events IEA Reactome
Glucagon signaling in metabolic regulation IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Integration of energy metabolism IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Metabolism IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
PKA activation IEA Reactome
PKA activation in glucagon signalling IEA Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Hedgehog IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome





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