Gene description for Ppm1a
Gene name protein phosphatase 1A, magnesium dependent, alpha isoform
Gene symbol Ppm1a
Other names/aliases 2310003C21Rik
2900017D14Rik
AI427932
AU017636
MMPa-2
MPPa-1
Species Mus musculus
 Database cross references - Ppm1a
ExoCarta ExoCarta_19042
Vesiclepedia VP_19042
Entrez Gene 19042
UniProt P49443  
 Ppm1a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppm1a
Molecular Function
    magnesium ion binding GO:0000287 IEA
    phosphoprotein phosphatase activity GO:0004721 IDA
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 ISO
    myosin phosphatase activity GO:0017018 IEA
    manganese ion binding GO:0030145 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 ISO
    transmembrane transporter binding GO:0044325 ISO
    R-SMAD binding GO:0070412 ISO
    R-SMAD binding GO:0070412 ISS
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 ISO
    N-terminal protein myristoylation GO:0006499 IDA
    protein export from nucleus GO:0006611 IMP
    dephosphorylation GO:0016311 ISO
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISO
    negative regulation of BMP signaling pathway GO:0030514 IMP
    peptidyl-threonine dephosphorylation GO:0035970 ISO
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISO
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISS
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of protein export from nucleus GO:0046827 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISO
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 IDA
    neuron projection GO:0043005 ISO
 Experiment description of studies that identified Ppm1a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppm1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pcdh9  
Affinity Capture-Western Mus musculus
2 Pcdh11x  
Affinity Capture-Western Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Pcdh1  
Affinity Capture-Western Mus musculus
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