Gene description for Reg3b
Gene name regenerating islet-derived 3 beta
Gene symbol Reg3b
Other names/aliases HIP
PAP1
Pap
REG-III
Species Mus musculus
 Database cross references - Reg3b
ExoCarta ExoCarta_18489
Vesiclepedia VP_18489
Entrez Gene 18489
UniProt P35230  
 Reg3b identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Reg3b
Molecular Function
    hormone activity GO:0005179 ISO
    growth factor binding GO:0019838 ISO
    carbohydrate binding GO:0030246 IEA
    signaling receptor activity GO:0038023 IBA
    identical protein binding GO:0042802 ISO
    peptidoglycan binding GO:0042834 IBA
    peptidoglycan binding GO:0042834 ISO
    metal ion binding GO:0046872 IEA
    oligosaccharide binding GO:0070492 IBA
    oligosaccharide binding GO:0070492 ISO
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    acute-phase response GO:0006953 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 ISO
    response to bacterium GO:0009617 IEP
    response to peptide hormone GO:0043434 IBA
    defense response to Gram-negative bacterium GO:0050829 IDA
    defense response to Gram-positive bacterium GO:0050830 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    negative regulation of cellular response to oxidative stress GO:1900408 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISO
    protein-containing complex GO:0032991 ISO
    zymogen granule GO:0042588 ISO
    apical part of cell GO:0045177 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Reg3b in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Reg3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Reg3b is involved
PathwayEvidenceSource
Antimicrobial peptides IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome





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