Gene description for P4ha1
Gene name procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide
Gene symbol P4ha1
Other names/aliases AL022634
P4ha
Species Mus musculus
 Database cross references - P4ha1
ExoCarta ExoCarta_18451
Vesiclepedia VP_18451
Entrez Gene 18451
UniProt Q60715  
 P4ha1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for P4ha1
Molecular Function
    procollagen-proline 4-dioxygenase activity GO:0004656 IBA
    procollagen-proline 4-dioxygenase activity GO:0004656 IDA
    procollagen-proline 4-dioxygenase activity GO:0004656 IMP
    procollagen-proline 4-dioxygenase activity GO:0004656 ISO
    iron ion binding GO:0005506 IEA
    L-ascorbic acid binding GO:0031418 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 IDA
    collagen fibril organization GO:0030199 IBA
    collagen fibril organization GO:0030199 IMP
Subcellular Localization
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    procollagen-proline 4-dioxygenase complex GO:0016222 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified P4ha1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for P4ha1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ero1l 50527
Co-fractionation Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Atxn1  
Proximity Label-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which P4ha1 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes IEA Reactome
Collagen formation IEA Reactome
Extracellular matrix organization IEA Reactome





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