Gene description for Nme2
Gene name NME/NM23 nucleoside diphosphate kinase 2
Gene symbol Nme2
Other names/aliases NM23-H2
NM23B
nm23-M2
Species Mus musculus
 Database cross references - Nme2
ExoCarta ExoCarta_18103
Vesiclepedia VP_18103
Entrez Gene 18103
UniProt Q01768  
 Nme2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nme2
Molecular Function
    phosphorelay sensor kinase activity GO:0000155 IEA
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 ISS
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    nucleoside diphosphate kinase activity GO:0004550 IBA
    nucleoside diphosphate kinase activity GO:0004550 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    fatty acid binding GO:0005504 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    intermediate filament binding GO:0019215 ISO
    enzyme binding GO:0019899 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    G-quadruplex DNA binding GO:0051880 ISO
    G-quadruplex DNA binding GO:0051880 ISS
    heterocyclic compound binding GO:1901363 ISO
Biological Process
    phosphorelay signal transduction system GO:0000160 IEA
    negative regulation of myeloid leukocyte differentiation GO:0002762 ISO
    GTP biosynthetic process GO:0006183 IEA
    GTP biosynthetic process GO:0006183 ISO
    UTP biosynthetic process GO:0006228 IEA
    UTP biosynthetic process GO:0006228 ISO
    dTMP biosynthetic process GO:0006231 NAS
    CTP biosynthetic process GO:0006241 IEA
    CTP biosynthetic process GO:0006241 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    integrin-mediated signaling pathway GO:0007229 ISS
    positive regulation of neuron projection development GO:0010976 ISO
    regulation of apoptotic process GO:0042981 IBA
    negative regulation of apoptotic process GO:0043066 IEA
    positive regulation of keratinocyte differentiation GO:0045618 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of epithelial cell proliferation GO:0050679 IEA
Subcellular Localization
    ruffle GO:0001726 ISO
    ruffle GO:0001726 ISS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HDA
    intermediate filament GO:0005882 ISO
    focal adhesion GO:0005925 ISS
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    mitochondrial membrane GO:0031966 ISO
    myelin sheath GO:0043209 HDA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell periphery GO:0071944 ISO
    cell periphery GO:0071944 ISS
 Experiment description of studies that identified Nme2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nme2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Nme3  
Co-fractionation Mus musculus
5 Taldo1 21351
Co-fractionation Mus musculus
6 MAPT  
Reconstituted Complex Homo sapiens
7 Idh1 15926
Co-fractionation Mus musculus
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