ExoCarta: Gene summary

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Gene description for Muc2
Gene name	mucin 2
Gene symbol	Muc2
Other names/aliases	2010015E03Rik MCM wnn
Species	Mus musculus
Database cross references - Muc2
ExoCarta	ExoCarta_17831
Vesiclepedia	VP_17831
Entrez Gene	17831
Muc2 identified in sEVs derived from the following tissue/cell type
Faeces	33431899
Faeces	33431899

Gene ontology annotations for Muc2

Molecular Function
	extracellular matrix structural constituent	GO:0005201	IDA
	protein binding	GO:0005515	IPI
	identical protein binding	GO:0042802	ISO
	cupric ion binding	GO:1903135	ISO
	cupric ion binding	GO:1903135	ISS
	cuprous ion binding	GO:1903136	ISO
	cuprous ion binding	GO:1903136	ISS
Biological Process
	glandular epithelial cell development	GO:0002068	IMP
	apoptotic process	GO:0006915	IMP
	negative regulation of cell population proliferation	GO:0008285	IMP
	detoxification of copper ion	GO:0010273	ISO
	detoxification of copper ion	GO:0010273	ISS
	maintenance of gastrointestinal epithelium	GO:0030277	IDA
	maintenance of gastrointestinal epithelium	GO:0030277	ISO
	negative regulation of cell migration	GO:0030336	IMP
	positive regulation of apoptotic process	GO:0043065	IMP
	host-mediated regulation of intestinal microbiota composition	GO:0048874	IDA
	mucus secretion	GO:0070254	IDA
	mucus secretion	GO:0070254	ISO
Subcellular Localization
	extracellular space	GO:0005615	IBA
	extracellular matrix	GO:0031012	IBA
	extracellular matrix	GO:0031012	IDA
	collagen-containing extracellular matrix	GO:0062023	HDA
	mucus layer	GO:0070701	IDA
	mucus layer	GO:0070701	ISO
	inner mucus layer	GO:0070702	IDA
	outer mucus layer	GO:0070703	IDA

Experiment description of studies that identified Muc2 in sEVs

Experiment ID

303

MISEV standards
✔ EM	Biophysical techniques
✔ Cd63\|Cd81\|Cd9	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33431899

Organism

Mus musculus

Experiment description

High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance

Authors

"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."

Journal name

Nat Commun

Publication year

2021

Sample

Faeces

Sample name

Intestinal epithelial cells - C57BL/6

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
Lipid
miRNA

Methods used in the study

Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC