Gene description for Mif
Gene name macrophage migration inhibitory factor
Gene symbol Mif
Other names/aliases GIF
Glif
Species Mus musculus
 Database cross references - Mif
ExoCarta ExoCarta_17319
Vesiclepedia VP_17319
Entrez Gene 17319
UniProt P34884  
 Mif identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mif
Molecular Function
    protease binding GO:0002020 IPI
    dopachrome isomerase activity GO:0004167 ISO
    dopachrome isomerase activity GO:0004167 ISS
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 ISO
    cytokine receptor binding GO:0005126 IEA
    cytokine receptor binding GO:0005126 ISO
    chemoattractant activity GO:0042056 IEA
    chemoattractant activity GO:0042056 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    phenylpyruvate tautomerase activity GO:0050178 IBA
    phenylpyruvate tautomerase activity GO:0050178 IMP
    phenylpyruvate tautomerase activity GO:0050178 ISO
Biological Process
    prostaglandin biosynthetic process GO:0001516 IEA
    prostaglandin biosynthetic process GO:0001516 ISO
    positive regulation of cytokine production GO:0001819 ISO
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of adaptive immune response GO:0002821 NAS
    negative regulation of mature B cell apoptotic process GO:0002906 IDA
    inflammatory response GO:0006954 IEA
    cell surface receptor signaling pathway GO:0007166 IEA
    cell surface receptor signaling pathway GO:0007166 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of protein kinase A signaling GO:0010739 ISO
    negative regulation of macrophage chemotaxis GO:0010760 IEA
    negative regulation of macrophage chemotaxis GO:0010760 ISO
    carboxylic acid metabolic process GO:0019752 ISO
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IMP
    negative regulation of cell migration GO:0030336 ISO
    positive regulation of B cell proliferation GO:0030890 IEA
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    negative regulation of myeloid cell apoptotic process GO:0033033 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    regulation of cell population proliferation GO:0042127 IMP
    positive regulation of phosphorylation GO:0042327 ISO
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of MAP kinase activity GO:0043406 IDA
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 IDA
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 ISO
    innate immune response GO:0045087 IEA
    positive regulation of fibroblast proliferation GO:0048146 IDA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    positive chemotaxis GO:0050918 IEA
    negative regulation of protein metabolic process GO:0051248 IDA
    positive regulation of prostaglandin secretion involved in immune response GO:0061078 IMP
    positive regulation of myeloid leukocyte cytokine production involved in immune response GO:0061081 IMP
    protein homotrimerization GO:0070207 IEA
    protein homotrimerization GO:0070207 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of arachidonate secretion GO:0090238 IDA
    cellular senescence GO:0090398 IMP
    positive regulation of cAMP/PKA signal transduction GO:0141163 IEA
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 IEA
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 ISO
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 IDA
    negative regulation of cellular senescence GO:2000773 IEA
    negative regulation of cellular senescence GO:2000773 ISO
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Mif in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mif
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Trp53  
Negative Genetic Mus musculus
2 VASP 7408
Affinity Capture-MS Homo sapiens
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