Gene description for Mid1
Gene name midline 1
Gene symbol Mid1
Other names/aliases 61B3-R
DXHXS1141
Fxy
Trim18
Species Mus musculus
 Database cross references - Mid1
ExoCarta ExoCarta_17318
Entrez Gene 17318
UniProt O70583  
 Mid1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Mid1
Molecular Function
    protein-glutamic acid ligase activity GO:0070739 IEA
    zinc ion binding GO:0008270 IEA
    metal ion binding GO:0046872 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    protein-glycine ligase activity GO:0070735 IEA
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    protein-glycine ligase activity, initiating GO:0070736 IEA
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    tubulin-glycine ligase activity GO:0070738 IEA
Biological Process
    negative regulation of microtubule depolymerization GO:0007026 IGI
Subcellular Localization
    microtubule cytoskeleton GO:0015630 IDA
    microtubule GO:0005874 IEA
    intracellular GO:0005622 IEA
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Mid1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Mid1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mid1 is involved
PathwayEvidenceSource
Interferon gamma signaling IEA Reactome





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