Gene description for Slc3a2
Gene name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Gene symbol Slc3a2
Other names/aliases 4F2
4F2HC
AI314110
Cd98
Ly-10
Ly-m10
Ly10
Mdu1
Mgp-2hc
NACAE
Species Mus musculus
 Database cross references - Slc3a2
ExoCarta ExoCarta_17254
Vesiclepedia VP_17254
Entrez Gene 17254
UniProt P10852  
 Slc3a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc3a2
Molecular Function
    double-stranded RNA binding GO:0003725 ISO
    neutral L-amino acid secondary active transmembrane transporter activity GO:0005294 ISO
    protein binding GO:0005515 IPI
    aromatic amino acid transmembrane transporter activity GO:0015173 IBA
    aromatic amino acid transmembrane transporter activity GO:0015173 IEA
    aromatic amino acid transmembrane transporter activity GO:0015173 ISO
    neutral L-amino acid transmembrane transporter activity GO:0015175 ISO
    L-alanine transmembrane transporter activity GO:0015180 IBA
    L-alanine transmembrane transporter activity GO:0015180 IEA
    L-alanine transmembrane transporter activity GO:0015180 ISO
    L-leucine transmembrane transporter activity GO:0015190 IBA
    L-leucine transmembrane transporter activity GO:0015190 IEA
    L-leucine transmembrane transporter activity GO:0015190 ISO
    protein homodimerization activity GO:0042803 IDA
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    exogenous protein binding GO:0140272 IEA
    exogenous protein binding GO:0140272 ISO
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    neutral amino acid transport GO:0015804 ISO
    isoleucine transport GO:0015818 IEA
    isoleucine transport GO:0015818 ISO
    L-leucine transport GO:0015820 IDA
    L-leucine transport GO:0015820 ISO
    L-leucine transport GO:0015820 ISS
    methionine transport GO:0015821 IEA
    methionine transport GO:0015821 ISO
    phenylalanine transport GO:0015823 IBA
    phenylalanine transport GO:0015823 IEA
    phenylalanine transport GO:0015823 ISO
    proline transport GO:0015824 IEA
    proline transport GO:0015824 ISO
    tryptophan transport GO:0015827 ISO
    tryptophan transport GO:0015827 ISS
    tyrosine transport GO:0015828 IEA
    tyrosine transport GO:0015828 ISO
    valine transport GO:0015829 IEA
    valine transport GO:0015829 ISO
    response to exogenous dsRNA GO:0043330 ISO
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    thyroid hormone transport GO:0070327 IEA
    thyroid hormone transport GO:0070327 ISO
    L-histidine transport GO:1902024 IEA
    L-histidine transport GO:1902024 ISO
    L-leucine import across plasma membrane GO:1903801 IBA
    L-leucine import across plasma membrane GO:1903801 ISO
    L-leucine import across plasma membrane GO:1903801 ISS
    L-alanine import across plasma membrane GO:1904273 IBA
    L-alanine import across plasma membrane GO:1904273 IEA
    L-alanine import across plasma membrane GO:1904273 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    lysosomal membrane GO:0005765 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basal plasma membrane GO:0009925 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    melanosome GO:0042470 IEA
    neuronal cell body GO:0043025 IDA
    apical pole of neuron GO:0044225 IDA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    anchoring junction GO:0070161 IEA
    amino acid transport complex GO:1990184 IEA
    amino acid transport complex GO:1990184 ISO
 Experiment description of studies that identified Slc3a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc3a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc1a2  
Affinity Capture-MS Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Sstr3  
Proximity Label-MS Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Kctd13  
Affinity Capture-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
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