Gene description for Mcm4
Gene name minichromosome maintenance deficient 4 homolog (S. cerevisiae)
Gene symbol Mcm4
Other names/aliases 19G
AI325074
AU045576
Cdc21
Mcmd4
mKIAA4003
mcdc21
Species Mus musculus
 Database cross references - Mcm4
ExoCarta ExoCarta_17217
Vesiclepedia VP_17217
Entrez Gene 17217
UniProt P49717  
 Mcm4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mcm4
Molecular Function
    DNA helicase activity GO:0003678 IDA
    DNA helicase activity GO:0003678 IMP
    DNA helicase activity GO:0003678 ISO
    DNA helicase activity GO:0003678 ISS
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IMP
    single-stranded DNA binding GO:0003697 IPI
    single-stranded DNA binding GO:0003697 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IBA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via break-induced replication GO:0000727 IBA
    DNA unwinding involved in DNA replication GO:0006268 IBA
    DNA unwinding involved in DNA replication GO:0006268 IPI
    DNA unwinding involved in DNA replication GO:0006268 ISO
    DNA replication initiation GO:0006270 IEA
    DNA strand elongation involved in DNA replication GO:0006271 IBA
    premeiotic DNA replication GO:0006279 NAS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
    mitotic DNA replication initiation GO:1902975 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    MCM complex GO:0042555 IBA
    MCM complex GO:0042555 IDA
    MCM complex GO:0042555 ISO
    MCM complex GO:0042555 ISS
    CMG complex GO:0071162 ISO
    CMG complex GO:0071162 ISS
 Experiment description of studies that identified Mcm4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mcm4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mcm7 17220
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Trp53  
Synthetic Growth Defect Mus musculus
4 Mcmbp  
Co-fractionation Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Cebpa  
Affinity Capture-MS Mus musculus
7 Atrx  
Co-localization Mus musculus
8 CDC7  
Biochemical Activity Homo sapiens
9 Mapk11  
Co-fractionation Mus musculus
10 Hells 15201
Co-fractionation Mus musculus
11 Mms22l  
Co-fractionation Mus musculus
12 Chaf1a  
Affinity Capture-MS Mus musculus
13 Orc3  
Co-fractionation Mus musculus
14 Cdc7  
Co-fractionation Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
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