Gene description for Mgst1
Gene name microsomal glutathione S-transferase 1
Gene symbol Mgst1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Mgst1
ExoCarta ExoCarta_171341
Vesiclepedia VP_171341
Entrez Gene 171341
UniProt P08011  
 Mgst1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Mgst1
Molecular Function
    glutathione transferase activity GO:0004364 IBA
    glutathione transferase activity GO:0004364 IDA
    glutathione transferase activity GO:0004364 IEA
    glutathione transferase activity GO:0004364 ISO
    glutathione transferase activity GO:0004364 TAS
    glutathione peroxidase activity GO:0004602 IEA
    glutathione peroxidase activity GO:0004602 ISO
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    glutathione binding GO:0043295 IDA
Biological Process
    glutathione metabolic process GO:0006749 ISO
    response to xenobiotic stimulus GO:0009410 IDA
    response to lipopolysaccharide GO:0032496 IDA
    Leydig cell differentiation GO:0033327 IEP
    glutathione transport GO:0034635 IEA
    glutathione transport GO:0034635 ISO
    cellular response to lipid hydroperoxide GO:0071449 IEA
    cellular response to lipid hydroperoxide GO:0071449 ISO
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IEA
    peroxisomal membrane GO:0005778 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 IDA
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified Mgst1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Mgst1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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