Gene description for Ehhadh
Gene name enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Gene symbol Ehhadh
Other names/aliases 1
Lbp
MEF
Mfe
Mfe1
Pbe
Pbfe
perMFE-1
Species Rattus norvegicus
 Database cross references - Ehhadh
ExoCarta ExoCarta_171142
Vesiclepedia VP_171142
Entrez Gene 171142
UniProt P07896  
 Ehhadh identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Ehhadh
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IBA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IDA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISO
    delta(3)-delta(2)-enoyl-CoA isomerase activity GO:0004165 IDA
    enoyl-CoA hydratase activity GO:0004300 IDA
    enoyl-CoA hydratase activity GO:0004300 ISO
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 ISO
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 ISS
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    lyase activity GO:0016829 IEA
    isomerase activity GO:0016853 IEA
    intramolecular oxidoreductase activity, transposing C=C bonds GO:0016863 IDA
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    NAD+ binding GO:0070403 IEA
Biological Process
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 IEA
    fatty acid beta-oxidation GO:0006635 ISO
    fatty acid beta-oxidation GO:0006635 ISO
Subcellular Localization
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Ehhadh in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Ehhadh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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