Gene description for Ehhadh
Gene name enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Gene symbol Ehhadh
Other names/aliases 1
Lbp
MEF
Mfe
Mfe1
Pbe
Pbfe
perMFE-1
Species Rattus norvegicus
 Database cross references - Ehhadh
ExoCarta ExoCarta_171142
Vesiclepedia VP_171142
Entrez Gene 171142
UniProt P07896  
 Ehhadh identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Ehhadh
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IBA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IDA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISO
    delta(3)-delta(2)-enoyl-CoA isomerase activity GO:0004165 IDA
    enoyl-CoA hydratase activity GO:0004300 IDA
    enoyl-CoA hydratase activity GO:0004300 ISO
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 ISO
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 ISS
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    lyase activity GO:0016829 IEA
    isomerase activity GO:0016853 IEA
    intramolecular oxidoreductase activity, transposing C=C bonds GO:0016863 IDA
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    NAD+ binding GO:0070403 IEA
Biological Process
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 IEA
    fatty acid beta-oxidation GO:0006635 ISO
    fatty acid beta-oxidation GO:0006635 ISO
Subcellular Localization
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Ehhadh in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Ehhadh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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