Experiment description of studies that identified Akap5 in sEVs

1 Experiment ID 92 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|GAPDH|CD9|CD151 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M." Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]

2 Experiment ID 93 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|GAPDH|CD9|CD151 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M." Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

Protein-protein interactions for Akap5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Prkch   Reconstituted Complex Rattus norvegicus 2 Prkci   Reconstituted Complex Rattus norvegicus 3 Prkcb   Reconstituted Complex Rattus norvegicus 4 Prkcg   Reconstituted Complex Rattus norvegicus 5 Prkca   Reconstituted Complex Rattus norvegicus 6 Adrb2   Affinity Capture-Western Rattus norvegicus Reconstituted Complex Rattus norvegicus 7 Prkcz   Reconstituted Complex Rattus norvegicus 8 Prkcd   Reconstituted Complex Rattus norvegicus 9 Trpv1   Affinity Capture-Western Rattus norvegicus 10 Prkce   Reconstituted Complex Rattus norvegicus View the network

Pathways in which Akap5 is involved

No pathways found