Gene description for Krt8
Gene name keratin 8
Gene symbol Krt8
Other names/aliases AA960620
AL022697
AU019895
Card2
EndoA
K8
Krt-2.8
Krt2-8
Species Mus musculus
 Database cross references - Krt8
ExoCarta ExoCarta_16691
Vesiclepedia VP_16691
Entrez Gene 16691
UniProt P11679  
 Krt8 identified in sEVs derived from the following tissue/cell type
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt8
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
Biological Process
    tumor necrosis factor-mediated signaling pathway GO:0033209 IGI
    tumor necrosis factor-mediated signaling pathway GO:0033209 IMP
    sarcomere organization GO:0045214 IEA
    sarcomere organization GO:0045214 ISO
    response to hydrostatic pressure GO:0051599 IEA
    response to hydrostatic pressure GO:0051599 ISO
    response to other organism GO:0051707 IMP
    cell differentiation involved in embryonic placenta development GO:0060706 IGI
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    hepatocyte apoptotic process GO:0097284 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    cytoplasm GO:0005737 ISO
    intermediate filament GO:0005882 IDA
    intermediate filament GO:0005882 ISO
    cell-cell junction GO:0005911 IDA
    dystrophin-associated glycoprotein complex GO:0016010 IEA
    dystrophin-associated glycoprotein complex GO:0016010 ISO
    apicolateral plasma membrane GO:0016327 IDA
    nuclear matrix GO:0016363 IEA
    Z disc GO:0030018 IDA
    Z disc GO:0030018 ISO
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    costamere GO:0043034 IEA
    costamere GO:0043034 ISO
    keratin filament GO:0045095 ISO
    intermediate filament cytoskeleton GO:0045111 ISO
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified Krt8 in sEVs
1
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa1b 15511
Affinity Capture-Western Mus musculus
2 Gfra1  
Co-fractionation Mus musculus
3 Qrsl1  
Co-fractionation Mus musculus
4 Psmc4 23996
Affinity Capture-Western Mus musculus
5 Nphp4  
Affinity Capture-MS Mus musculus
6 Krt18 16668
Co-fractionation Mus musculus
7 Dsp 109620
Co-fractionation Mus musculus
8 Slc9a1  
Co-fractionation Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Thrb  
Affinity Capture-MS Mus musculus
11 Prrt1  
Co-fractionation Mus musculus
12 Psmb5 19173
Affinity Capture-Western Mus musculus
13 Pkp1  
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Zdhhc5 228136
Co-fractionation Mus musculus
16 Hsp90aa1 15519
Affinity Capture-Western Mus musculus
17 Tgm2  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Krt8 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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