|
 Gene description for DEFA3 |
| Gene name |
defensin, alpha 3, neutrophil-specific |
| Gene symbol |
DEFA3 |
| Other names/aliases |
DEF3
HNP-3
HNP3
HP-3
HP3 |
| Species |
Homo sapiens |
| Database cross references - DEFA3 |
| ExoCarta |
ExoCarta_1668 |
| Vesiclepedia |
VP_1668 |
| Entrez Gene |
1668 |
| HGNC |
2762 |
| MIM |
604522 |
| UniProt |
P59666
|
| DEFA3 identified in sEVs derived from the following tissue/cell type |
|
Chondrocytes
|
35931686
|
|
Chondrocytes
|
35931686
|
|
Urine
|
15326289
|
| Gene ontology annotations for DEFA3 |
|
|
| Experiment description of studies that identified DEFA3 in sEVs |
| 1 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 2 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 3 |
| Experiment ID |
13 |
| MISEV standards |
|
✔
IEM
|
Biophysical techniques |
|
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
15326289
|
| Organism |
Homo sapiens |
| Experiment description |
Identification and proteomic profiling of exosomes in human urine. |
| Authors |
"Pisitkun T, Shen RF, Knepper MA" |
| Journal name |
PNAS
|
| Publication year |
2004 |
| Sample |
Urine |
| Sample name |
Urine - Normal |
| Isolation/purification methods |
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA XP]
Western blotting |
|
|
| Protein-protein interactions for DEFA3 |
|
|
| Pathways in which DEFA3 is involved |
|
|
|
