Gene description for Itm2b
Gene name integral membrane protein 2B
Gene symbol Itm2b
Other names/aliases AI256040
Bri
Bri2
Bricd2b
D14Sel6
E25BMM
imBRI2
Species Mus musculus
 Database cross references - Itm2b
ExoCarta ExoCarta_16432
Vesiclepedia VP_16432
Entrez Gene 16432
UniProt O89051  
 Itm2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itm2b
Molecular Function
    amyloid-beta binding GO:0001540 IBA
    amyloid-beta binding GO:0001540 IEA
    amyloid-beta binding GO:0001540 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
Biological Process
    negative regulation of amyloid precursor protein biosynthetic process GO:0042985 IBA
    negative regulation of amyloid precursor protein biosynthetic process GO:0042985 ISO
    negative regulation of amyloid precursor protein biosynthetic process GO:0042985 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    endosome membrane GO:0010008 IEA
    Golgi-associated vesicle membrane GO:0030660 ISO
    Golgi-associated vesicle membrane GO:0030660 ISS
    organelle membrane GO:0031090 ISO
    organelle membrane GO:0031090 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Itm2b in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itm2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ngly1  
Co-fractionation Mus musculus
2 Nphs2  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Lmo2  
Two-hybrid Mus musculus
5 Tmem38b  
Co-fractionation Mus musculus
6 Sepp1  
Co-fractionation Mus musculus
7 Unk  
Affinity Capture-RNA Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Itm2b is involved
No pathways found





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