Gene description for Ide
Gene name insulin degrading enzyme
Gene symbol Ide
Other names/aliases 1300012G03Rik
4833415K22Rik
AA675336
AI507533
Species Mus musculus
 Database cross references - Ide
ExoCarta ExoCarta_15925
Vesiclepedia VP_15925
Entrez Gene 15925
 Ide identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ide
Molecular Function
    amyloid-beta binding GO:0001540 ISO
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 ISO
    endopeptidase activity GO:0004175 ISS
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 ISO
    metalloendopeptidase activity GO:0004222 ISS
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    ATP hydrolysis activity GO:0016887 ISO
    peptide hormone binding GO:0017046 ISO
    beta-endorphin binding GO:0031626 ISO
    peptide binding GO:0042277 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    insulin binding GO:0043559 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    proteolysis GO:0006508 IEA
    proteolysis GO:0006508 ISO
    response to oxidative stress GO:0006979 IDA
    bradykinin catabolic process GO:0010815 ISO
    bradykinin catabolic process GO:0010815 ISS
    ubiquitin recycling GO:0010992 ISO
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 ISO
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 ISS
    protein catabolic process GO:0030163 IMP
    protein catabolic process GO:0030163 ISO
    protein catabolic process GO:0030163 ISS
    positive regulation of protein binding GO:0032092 ISO
    insulin receptor recycling GO:0038020 IDA
    hormone catabolic process GO:0042447 IBA
    hormone catabolic process GO:0042447 ISO
    hormone catabolic process GO:0042447 ISS
    peptide catabolic process GO:0043171 IBA
    peptide catabolic process GO:0043171 ISO
    peptide catabolic process GO:0043171 ISS
    negative regulation of proteolysis GO:0045861 ISO
    amyloid-beta metabolic process GO:0050435 IBA
    amyloid-beta metabolic process GO:0050435 ISO
    amyloid-beta metabolic process GO:0050435 ISS
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 ISO
    amyloid-beta clearance GO:0097242 IDA
    amyloid-beta clearance GO:0097242 IMP
    amyloid-beta clearance GO:0097242 ISO
    amyloid-beta clearance by cellular catabolic process GO:0150094 ISO
    insulin metabolic process GO:1901142 ISO
    insulin catabolic process GO:1901143 IDA
    insulin catabolic process GO:1901143 IMP
    insulin catabolic process GO:1901143 ISO
    insulin catabolic process GO:1901143 ISS
    regulation of aerobic respiration GO:1903715 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    peroxisome GO:0005777 ISO
    peroxisomal matrix GO:0005782 IBA
    peroxisomal matrix GO:0005782 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IEA
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 ISO
    basolateral plasma membrane GO:0016323 ISO
    cytosolic proteasome complex GO:0031597 ISO
    endosome lumen GO:0031904 IDA
    extracellular exosome GO:0070062 IMP
 Experiment description of studies that identified Ide in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ide
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ide is involved
No pathways found





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