Gene description for Hprt
Gene name hypoxanthine guanine phosphoribosyl transferase
Gene symbol Hprt
Other names/aliases C81579
HPGRT
Hprt1
Species Mus musculus
 Database cross references - Hprt
ExoCarta ExoCarta_15452
Vesiclepedia VP_15452
Entrez Gene 15452
UniProt P00493  
 Hprt identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hprt
Molecular Function
    nucleotide binding GO:0000166 IEA
    magnesium ion binding GO:0000287 IBA
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    hypoxanthine phosphoribosyltransferase activity GO:0004422 IBA
    hypoxanthine phosphoribosyltransferase activity GO:0004422 IDA
    hypoxanthine phosphoribosyltransferase activity GO:0004422 IMP
    hypoxanthine phosphoribosyltransferase activity GO:0004422 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    guanine phosphoribosyltransferase activity GO:0052657 IMP
    guanine phosphoribosyltransferase activity GO:0052657 ISO
    guanine phosphoribosyltransferase activity GO:0052657 ISS
Biological Process
    T cell mediated cytotoxicity GO:0001913 IMP
    response to amphetamine GO:0001975 IMP
    purine nucleotide biosynthetic process GO:0006164 ISO
    purine ribonucleoside salvage GO:0006166 IGI
    purine ribonucleoside salvage GO:0006166 IMP
    purine ribonucleoside salvage GO:0006166 ISO
    guanine salvage GO:0006178 IBA
    guanine salvage GO:0006178 IEA
    guanine salvage GO:0006178 ISO
    grooming behavior GO:0007625 IGI
    grooming behavior GO:0007625 IGI
    locomotory behavior GO:0007626 IMP
    striatum development GO:0021756 IMP
    cerebral cortex neuron differentiation GO:0021895 IMP
    central nervous system neuron development GO:0021954 IMP
    GMP salvage GO:0032263 IBA
    GMP salvage GO:0032263 IMP
    GMP salvage GO:0032263 IMP
    GMP salvage GO:0032263 ISO
    IMP salvage GO:0032264 IBA
    IMP salvage GO:0032264 IEA
    IMP salvage GO:0032264 IMP
    dopamine metabolic process GO:0042417 IMP
    hypoxanthine salvage GO:0043103 ISO
    hypoxanthine salvage GO:0043103 ISS
    AMP salvage GO:0044209 IDA
    positive regulation of dopamine metabolic process GO:0045964 IEA
    positive regulation of dopamine metabolic process GO:0045964 ISO
    GMP catabolic process GO:0046038 ISO
    GMP catabolic process GO:0046038 ISS
    IMP metabolic process GO:0046040 ISO
    IMP metabolic process GO:0046040 ISS
    adenine metabolic process GO:0046083 IGI
    hypoxanthine metabolic process GO:0046100 IBA
    hypoxanthine metabolic process GO:0046100 IC
    hypoxanthine metabolic process GO:0046100 IMP
    hypoxanthine metabolic process GO:0046100 ISO
    lymphocyte proliferation GO:0046651 IMP
    dendrite morphogenesis GO:0048813 IMP
    protein homotetramerization GO:0051289 IEA
    protein homotetramerization GO:0051289 ISO
    dopaminergic neuron differentiation GO:0071542 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Hprt in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hprt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tia1  
Affinity Capture-MS Mus musculus
2 Mapt  
Affinity Capture-MS Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
5 Wdyhv1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Hprt is involved
PathwayEvidenceSource
Azathioprine ADME IEA Reactome
Drug ADME IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide salvage IEA Reactome
Purine salvage IEA Reactome





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