Gene description for Hcls1
Gene name hematopoietic cell specific Lyn substrate 1
Gene symbol Hcls1
Other names/aliases AW213261
HS1
Species Mus musculus
 Database cross references - Hcls1
ExoCarta ExoCarta_15163
Vesiclepedia VP_15163
Entrez Gene 15163
UniProt P49710  
 Hcls1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hcls1
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IDA
    SH3 domain binding GO:0017124 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    signaling adaptor activity GO:0035591 IEA
    signaling adaptor activity GO:0035591 ISO
    protein-containing complex binding GO:0044877 IPI
    actin filament binding GO:0051015 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    actin filament organization GO:0007015 IEA
    actin filament organization GO:0007015 ISO
    positive regulation of cell population proliferation GO:0008284 IMP
    response to hormone GO:0009725 IDA
    cell migration GO:0016477 IBA
    erythrocyte differentiation GO:0030218 IMP
    regulation of actin filament polymerization GO:0030833 IBA
    regulation of actin filament polymerization GO:0030833 IEA
    regulation of actin filament polymerization GO:0030833 ISO
    positive regulation of granulocyte differentiation GO:0030854 IMP
    positive regulation of granulocyte differentiation GO:0030854 ISO
    granulocyte colony-stimulating factor signaling pathway GO:0038158 IEA
    granulocyte colony-stimulating factor signaling pathway GO:0038158 ISO
    positive regulation of protein import into nucleus GO:0042307 IEA
    positive regulation of protein import into nucleus GO:0042307 ISO
    positive regulation of macrophage differentiation GO:0045651 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    nuclear transport GO:0051169 IEA
    nuclear transport GO:0051169 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    cellular response to cytokine stimulus GO:0071345 ISO
    negative regulation of leukocyte apoptotic process GO:2000107 IEA
    negative regulation of leukocyte apoptotic process GO:2000107 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    transcription regulator complex GO:0005667 IEA
    transcription regulator complex GO:0005667 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    actin filament GO:0005884 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    site of polarized growth GO:0030427 IBA
    cortical actin cytoskeleton GO:0030864 IBA
 Experiment description of studies that identified Hcls1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hcls1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nfe2  
Two-hybrid Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Hs1bp3  
Affinity Capture-Western Mus musculus
Protein-peptide Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Hcls1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here