Gene description for Got1
Gene name glutamic-oxaloacetic transaminase 1, soluble
Gene symbol Got1
Other names/aliases AI789014
Got-1
cAspAT
cCAT
Species Mus musculus
 Database cross references - Got1
ExoCarta ExoCarta_14718
Vesiclepedia VP_14718
Entrez Gene 14718
UniProt P05201  
 Got1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Got1
Molecular Function
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IBA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IDA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISO
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISS
    phosphatidylserine decarboxylase activity GO:0004609 IDA
    pyridoxal phosphate binding GO:0030170 IEA
    carboxylic acid binding GO:0031406 ISO
    L-cysteine transaminase activity GO:0047801 ISO
    L-cysteine transaminase activity GO:0047801 ISS
Biological Process
    gluconeogenesis GO:0006094 IDA
    gluconeogenesis GO:0006094 ISO
    2-oxoglutarate metabolic process GO:0006103 ISS
    oxaloacetate metabolic process GO:0006107 IDA
    oxaloacetate metabolic process GO:0006107 ISO
    glycerol biosynthetic process GO:0006114 IDA
    glycerol biosynthetic process GO:0006114 ISO
    aspartate metabolic process GO:0006531 ISO
    aspartate metabolic process GO:0006531 ISS
    aspartate biosynthetic process GO:0006532 IBA
    aspartate biosynthetic process GO:0006532 IDA
    aspartate catabolic process GO:0006533 IEA
    aspartate catabolic process GO:0006533 ISO
    glutamate metabolic process GO:0006536 ISS
    Notch signaling pathway GO:0007219 IDA
    glutamate catabolic process to aspartate GO:0019550 IDA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISO
    cellular response to insulin stimulus GO:0032869 IEA
    negative regulation of collagen biosynthetic process GO:0032966 ISO
    malate-aspartate shuttle GO:0043490 IMP
    dicarboxylic acid metabolic process GO:0043648 ISO
    response to glucocorticoid GO:0051384 IEA
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISO
    negative regulation of mitochondrial depolarization GO:0051902 ISO
    fatty acid homeostasis GO:0055089 IDA
    transdifferentiation GO:0060290 ISO
    cellular response to mechanical stimulus GO:0071260 ISO
    response to transition metal nanoparticle GO:1990267 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    axon terminus GO:0043679 ISO
 Experiment description of studies that identified Got1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Got1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 MAPT  
Reconstituted Complex Homo sapiens
4 Tia1  
Affinity Capture-MS Mus musculus
5 Actn2  
Co-fractionation Mus musculus
6 Kcnma1  
Affinity Capture-MS Mus musculus
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