Gene description for Gng2
Gene name guanine nucleotide binding protein (G protein), gamma 2
Gene symbol Gng2
Other names/aliases 82
Species Mus musculus
 Database cross references - Gng2
ExoCarta ExoCarta_14702
Vesiclepedia VP_14702
Entrez Gene 14702
UniProt P63213  
 Gng2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gng2
Molecular Function
    GTPase activity GO:0003924 ISO
    G-protein beta-subunit binding GO:0031681 IBA
    G-protein beta-subunit binding GO:0031681 ISO
    G-protein beta-subunit binding GO:0031681 ISS
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    fibroblast proliferation GO:0048144 IDA
Subcellular Localization
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 ISO
    plasma membrane GO:0005886 TAS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Gng2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gng2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Gng2 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels IEA Reactome
Activation of GABAB receptors IEA Reactome
Activation of kainate receptors upon glutamate binding IEA Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
ADP signalling through P2Y purinoceptor 12 IEA Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Aquaporin-mediated transport IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Class B/2 (Secretin family receptors) IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
ESR-mediated signaling IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
G alpha (12/13) signalling events IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome
G beta:gamma signalling through BTK IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G beta:gamma signalling through PLC beta IEA Reactome
G protein gated Potassium channels IEA Reactome
G-protein activation IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GABA B receptor activation IEA Reactome
GABA receptor activation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors IEA Reactome
GPCR downstream signalling IEA Reactome
GPCR ligand binding IEA Reactome
GPER1 signaling IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits IEA Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels IEA Reactome
Metabolism IEA Reactome
Metabolism of proteins IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Potassium Channels IEA Reactome
Presynaptic function of Kainate receptors IEA Reactome
Prostacyclin signalling through prostacyclin receptor IEA Reactome
Protein folding IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal amplification IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by WNT IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome





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