Gene description for Gnai2
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting 2
Gene symbol Gnai2
Other names/aliases C76432
Galphai2
Gia
Gnai-2
Species Mus musculus
 Database cross references - Gnai2
ExoCarta ExoCarta_14678
Vesiclepedia VP_14678
Entrez Gene 14678
UniProt P08752  
 Gnai2 identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Dendritic cells 10545503    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnai2
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    G protein-coupled adenosine receptor signaling pathway GO:0001973 IBA
    G protein-coupled adenosine receptor signaling pathway GO:0001973 IEA
    G protein-coupled adenosine receptor signaling pathway GO:0001973 ISO
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IEA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IBA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IMP
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISA
    G protein-coupled acetylcholine receptor signaling pathway GO:0007213 IMP
    gamma-aminobutyric acid signaling pathway GO:0007214 IBA
    gamma-aminobutyric acid signaling pathway GO:0007214 IEA
    gamma-aminobutyric acid signaling pathway GO:0007214 ISO
    regulation of heart contraction GO:0008016 IMP
    cell population proliferation GO:0008283 IPI
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of superoxide anion generation GO:0032930 IEA
    positive regulation of superoxide anion generation GO:0032930 ISO
    positive regulation of urine volume GO:0035810 IEA
    positive regulation of urine volume GO:0035810 ISO
    negative regulation of calcium ion-dependent exocytosis GO:0045955 IEA
    negative regulation of calcium ion-dependent exocytosis GO:0045955 ISO
    positive regulation of insulin receptor signaling pathway GO:0046628 IEA
    positive regulation of insulin receptor signaling pathway GO:0046628 ISO
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    negative regulation of synaptic transmission GO:0050805 IEA
    negative regulation of synaptic transmission GO:0050805 ISO
    cell division GO:0051301 ISO
    cell division GO:0051301 ISS
    regulation of calcium ion transport GO:0051924 IEA
    regulation of calcium ion transport GO:0051924 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071878 IEA
    negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071878 ISO
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 ISO
    positive regulation of neural precursor cell proliferation GO:2000179 IEA
    positive regulation of neural precursor cell proliferation GO:2000179 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    dendrite GO:0030425 IDA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cell body GO:0044297 IDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IDA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IMP
    neuronal dense core vesicle GO:0098992 EXP
    neuronal dense core vesicle GO:0098992 IDA
 Experiment description of studies that identified Gnai2 in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
4
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
5
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnai2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Foxl2  
Two-hybrid Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Pcp2  
Affinity Capture-Western Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Nans 94181
Co-fractionation Mus musculus
8 Psat1 107272
Co-fractionation Mus musculus
9 Tuba4a 22145
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Gdi1 14567
Co-fractionation Mus musculus
12 Scrn1  
Co-fractionation Mus musculus
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