Gene description for Slc6a9
Gene name solute carrier family 6 (neurotransmitter transporter, glycine), member 9
Gene symbol Slc6a9
Other names/aliases Glyt-1
Glyt1
Species Mus musculus
 Database cross references - Slc6a9
ExoCarta ExoCarta_14664
Vesiclepedia VP_14664
Entrez Gene 14664
UniProt P28571  
 Slc6a9 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc6a9
Molecular Function
    amino acid:sodium symporter activity GO:0005283 IBA
    glycine transmembrane transporter activity GO:0015187 IDA
    glycine transmembrane transporter activity GO:0015187 IMP
    glycine transmembrane transporter activity GO:0015187 ISO
    glycine:sodium symporter activity GO:0015375 IDA
    glycine:sodium symporter activity GO:0015375 ISO
Biological Process
    neurotransmitter uptake GO:0001504 IDA
    neurotransmitter uptake GO:0001504 IMP
    glycine transport GO:0015816 IDA
    glycine transport GO:0015816 IMP
    sodium ion transmembrane transport GO:0035725 IBA
    sodium ion transmembrane transport GO:0035725 IEA
    positive regulation of hemoglobin biosynthetic process GO:0046985 IMP
    regulation of synaptic transmission, glycinergic GO:0060092 IMP
    glycine secretion, neurotransmission GO:0061537 IMP
    positive regulation of heme biosynthetic process GO:0070455 IMP
    glycine import across plasma membrane GO:1903804 IBA
    glycine import across plasma membrane GO:1903804 IMP
    glycine import across plasma membrane GO:1903804 ISO
    negative regulation of NMDA glutamate receptor activity GO:1904782 IMP
Subcellular Localization
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    basal plasma membrane GO:0009925 ISO
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 IC
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 ISO
    synaptic vesicle membrane GO:0030672 ISO
    dense core granule GO:0031045 ISO
    asymmetric synapse GO:0032279 ISO
    presynaptic membrane GO:0042734 ISO
    postsynaptic membrane GO:0045211 ISO
    hippocampal mossy fiber to CA3 synapse GO:0098686 ISO
    parallel fiber to Purkinje cell synapse GO:0098688 ISO
    presynapse GO:0098793 IEA
 Experiment description of studies that identified Slc6a9 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc6a9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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