Gene description for Galk1
Gene name galactokinase 1
Gene symbol Galk1
Other names/aliases AA409894
GALK
Gk
Glk
Glk1
Species Mus musculus
 Database cross references - Galk1
ExoCarta ExoCarta_14635
Vesiclepedia VP_14635
Entrez Gene 14635
UniProt Q9R0N0  
 Galk1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Galk1
Molecular Function
    galactokinase activity GO:0004335 IBA
    galactokinase activity GO:0004335 IDA
    galactokinase activity GO:0004335 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    galactose binding GO:0005534 IEA
    galactose binding GO:0005534 ISO
Biological Process
    galactose metabolic process GO:0006012 IBA
    galactose metabolic process GO:0006012 IDA
    galactose metabolic process GO:0006012 IMP
    galactose metabolic process GO:0006012 ISO
    galactitol metabolic process GO:0019402 IMP
    galactose catabolic process via UDP-galactose GO:0033499 IC
    galactose catabolic process via UDP-galactose GO:0033499 IMP
    carbohydrate phosphorylation GO:0046835 IEA
    carbohydrate phosphorylation GO:0046835 ISO
    glycolytic process from galactose GO:0061623 IC
    glycolytic process from galactose GO:0061623 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Galk1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Galk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Galk1 is involved
PathwayEvidenceSource
Galactose catabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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