Gene description for Gclc
Gene name glutamate-cysteine ligase, catalytic subunit
Gene symbol Gclc
Other names/aliases D9Wsu168e
GLCL-H
Ggcs-hs
Glclc
Species Mus musculus
 Database cross references - Gclc
ExoCarta ExoCarta_14629
Vesiclepedia VP_14629
Entrez Gene 14629
UniProt P97494  
 Gclc identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gclc
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    glutamate-cysteine ligase activity GO:0004357 IBA
    glutamate-cysteine ligase activity GO:0004357 IDA
    glutamate-cysteine ligase activity GO:0004357 IMP
    glutamate-cysteine ligase activity GO:0004357 ISO
    glutamate-cysteine ligase activity GO:0004357 ISS
    protein binding GO:0005515 IPI
    protein binding GO:0005515 ISS
    ATP binding GO:0005524 IEA
    glutamate binding GO:0016595 ISO
    glutamate binding GO:0016595 ISS
    ADP binding GO:0043531 ISO
    ADP binding GO:0043531 ISS
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    cysteine metabolic process GO:0006534 ISO
    cysteine metabolic process GO:0006534 ISS
    glutamate metabolic process GO:0006536 ISO
    glutamate metabolic process GO:0006536 ISS
    glutathione metabolic process GO:0006749 IMP
    glutathione biosynthetic process GO:0006750 IBA
    glutathione biosynthetic process GO:0006750 IDA
    glutathione biosynthetic process GO:0006750 IEA
    glutathione biosynthetic process GO:0006750 IMP
    glutathione biosynthetic process GO:0006750 ISO
    glutathione biosynthetic process GO:0006750 ISS
    response to oxidative stress GO:0006979 ISO
    response to oxidative stress GO:0006979 ISS
    response to nutrient GO:0007584 IEA
    response to heat GO:0009408 ISO
    response to heat GO:0009408 ISS
    response to xenobiotic stimulus GO:0009410 IMP
    response to hormone GO:0009725 ISO
    response to hormone GO:0009725 ISS
    response to activity GO:0014823 IEA
    L-ascorbic acid metabolic process GO:0019852 IMP
    negative regulation of protein ubiquitination GO:0031397 IMP
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    cellular response to insulin stimulus GO:0032869 IEA
    cellular response to hepatocyte growth factor stimulus GO:0035729 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    negative regulation of neuron apoptotic process GO:0043524 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    response to human chorionic gonadotropin GO:0044752 IEA
    cell redox homeostasis GO:0045454 ISO
    cell redox homeostasis GO:0045454 ISS
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    response to arsenic-containing substance GO:0046685 IMP
    response to cadmium ion GO:0046686 IEA
    response to nitrosative stress GO:0051409 IEA
    response to nitrosative stress GO:0051409 ISO
    regulation of mitochondrial depolarization GO:0051900 IGI
    response to interleukin-1 GO:0070555 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to follicle-stimulating hormone stimulus GO:0071372 IEA
    cellular response to thyroxine stimulus GO:0097069 IEA
    blood vessel diameter maintenance GO:0097746 ISO
    blood vessel diameter maintenance GO:0097746 ISS
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IGI
    negative regulation of hepatic stellate cell activation GO:2000490 IEA
    negative regulation of hepatic stellate cell activation GO:2000490 ISO
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IGI
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    glutamate-cysteine ligase complex GO:0017109 IBA
    glutamate-cysteine ligase complex GO:0017109 IDA
    glutamate-cysteine ligase complex GO:0017109 ISO
 Experiment description of studies that identified Gclc in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gclc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gclm  
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
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