Gene description for Gfpt1
Gene name glutamine fructose-6-phosphate transaminase 1
Gene symbol Gfpt1
Other names/aliases 2810423A18Rik
AI324119
AI449986
GFA
GFAT
GFAT1
GFAT1m
Gfpt
Species Mus musculus
 Database cross references - Gfpt1
ExoCarta ExoCarta_14583
Vesiclepedia VP_14583
Entrez Gene 14583
UniProt P47856  
 Gfpt1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gfpt1
Molecular Function
    glutamine-fructose-6-phosphate transaminase (isomerizing) activity GO:0004360 IBA
    glutamine-fructose-6-phosphate transaminase (isomerizing) activity GO:0004360 IEA
    glutamine-fructose-6-phosphate transaminase (isomerizing) activity GO:0004360 ISO
    amino acid binding GO:0016597 ISO
    carbohydrate binding GO:0030246 ISO
    identical protein binding GO:0042802 ISO
    carbohydrate derivative binding GO:0097367 IEA
Biological Process
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IEA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    glucosamine biosynthetic process GO:0006042 ISO
    UDP-N-acetylglucosamine metabolic process GO:0006047 IBA
    UDP-N-acetylglucosamine metabolic process GO:0006047 ISO
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 IEA
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 ISO
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 ISS
    protein N-linked glycosylation GO:0006487 IBA
    circadian regulation of gene expression GO:0032922 IMP
    negative regulation of glycogen biosynthetic process GO:0045719 ISO
 Experiment description of studies that identified Gfpt1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gfpt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Fitm2  
Positive Genetic Mus musculus
5 Unk  
Affinity Capture-RNA Mus musculus
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