Gene description for Gamt
Gene name guanidinoacetate methyltransferase
Gene symbol Gamt
Other names/aliases AA571402
Spintz1
Species Mus musculus
 Database cross references - Gamt
ExoCarta ExoCarta_14431
Vesiclepedia VP_14431
Entrez Gene 14431
UniProt O35969  
 Gamt identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gamt
Molecular Function
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 ISO
    guanidinoacetate N-methyltransferase activity GO:0030731 IBA
    guanidinoacetate N-methyltransferase activity GO:0030731 ISO
    guanidinoacetate N-methyltransferase activity GO:0030731 ISS
    identical protein binding GO:0042802 ISO
Biological Process
    creatine biosynthetic process GO:0006601 IBA
    creatine biosynthetic process GO:0006601 IEA
    creatine biosynthetic process GO:0006601 IMP
    creatine biosynthetic process GO:0006601 ISO
    spermatogenesis GO:0007283 IMP
    animal organ morphogenesis GO:0009887 IMP
    methylation GO:0032259 IEA
    regulation of multicellular organism growth GO:0040014 IMP
    S-adenosylhomocysteine metabolic process GO:0046498 ISO
    S-adenosylmethionine metabolic process GO:0046500 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    microvillus GO:0005902 IEA
 Experiment description of studies that identified Gamt in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gamt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gamt is involved
PathwayEvidenceSource
Creatine metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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