Gene description for Ganab
Gene name alpha glucosidase 2 alpha neutral subunit
Gene symbol Ganab
Other names/aliases AU042638
G2an
GluII
mKIAA0088
Species Mus musculus
 Database cross references - Ganab
ExoCarta ExoCarta_14376
Vesiclepedia VP_14376
Entrez Gene 14376
UniProt Q8BHN3  
 Ganab identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ganab
Molecular Function
    protein binding GO:0005515 IPI
    glucosidase activity GO:0015926 ISO
    carbohydrate binding GO:0030246 IEA
    glucan 1,3-alpha-glucosidase activity GO:0033919 IBA
    glucan 1,3-alpha-glucosidase activity GO:0033919 IDA
    glucan 1,3-alpha-glucosidase activity GO:0033919 ISO
    glucan 1,3-alpha-glucosidase activity GO:0033919 ISS
    alpha-glucosidase activity GO:0090599 ISO
    Glc2Man9GlcNAc2 oligosaccharide glucosidase activity GO:0106407 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    N-glycan processing GO:0006491 IBA
    N-glycan processing GO:0006491 IDA
    N-glycan processing GO:0006491 ISO
    N-glycan processing GO:0006491 ISS
    N-glycan processing GO:0006491 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 TAS
    Golgi apparatus GO:0005794 IEA
    glucosidase II complex GO:0017177 IBA
    glucosidase II complex GO:0017177 IDA
    glucosidase II complex GO:0017177 IPI
    glucosidase II complex GO:0017177 ISO
    melanosome GO:0042470 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Ganab in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ganab
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg12  
Negative Genetic Mus musculus
2 Olfm1  
Affinity Capture-MS Mus musculus
3 Lipc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ganab is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here