Gene description for Fth1
Gene name ferritin heavy chain 1
Gene symbol Fth1
Other names/aliases FHC
Fth
HFt
MFH
Species Mus musculus
 Database cross references - Fth1
ExoCarta ExoCarta_14319
Entrez Gene 14319
UniProt P09528  
 Fth1 identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Fth1
Molecular Function
    ferroxidase activity GO:0004322 IEA
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    ferric iron binding GO:0008199 IEA
Biological Process
    negative regulation of fibroblast proliferation GO:0048147 ISO
    intracellular sequestering of iron ion GO:0006880 ISO
    cellular iron ion homeostasis GO:0006879 IEA
    oxidation-reduction process GO:0055114 IEA
    iron ion transport GO:0006826 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Fth1 in exosomes
1
Experiment ID 26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Fth1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Fth1 is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis IEA Reactome
Iron uptake and transport IEA Reactome
Scavenging by Class A Receptors IEA Reactome





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