Gene description for Arf3
Gene name ADP-ribosylation factor 3
Gene symbol Arf3
Other names/aliases Ac1-253
Species Rattus norvegicus
 Database cross references - Arf3
ExoCarta ExoCarta_140940
Vesiclepedia VP_140940
Entrez Gene 140940
UniProt P61206  
 Arf3 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Arf3
Molecular Function
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Arf3 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Arf3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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