Gene description for F5
Gene name coagulation factor V
Gene symbol F5
Other names/aliases AI173222
Cf-5
Cf5
Species Mus musculus
 Database cross references - F5
ExoCarta ExoCarta_14067
Vesiclepedia VP_14067
Entrez Gene 14067
UniProt O88783  
 F5 identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for F5
Molecular Function
    copper ion binding GO:0005507 IEA
Biological Process
    blood coagulation GO:0007596 IBA
    blood coagulation GO:0007596 IDA
    blood coagulation GO:0007596 IMP
    blood circulation GO:0008015 IBA
    blood circulation GO:0008015 IMP
    response to vitamin K GO:0032571 IEA
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    platelet alpha granule GO:0031091 IBA
    platelet alpha granule GO:0031091 IDA
 Experiment description of studies that identified F5 in sEVs
1
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for F5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Serpina3c  
Co-fractionation Mus musculus
2 Mmrn1  
Co-fractionation Mus musculus
3 Ctsg  
Co-fractionation Mus musculus
View the network image/svg+xml



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