Gene description for F3
Gene name coagulation factor III
Gene symbol F3
Other names/aliases AA409063
CD142
Cf-3
Cf3
TF
Species Mus musculus
 Database cross references - F3
ExoCarta ExoCarta_14066
Vesiclepedia VP_14066
Entrez Gene 14066
UniProt P20352  
 F3 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for F3
Molecular Function
    protease binding GO:0002020 ISO
    serine-type endopeptidase activity GO:0004252 ISO
    cytokine receptor activity GO:0004896 IBA
    phospholipid binding GO:0005543 ISO
Biological Process
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    activation of plasma proteins involved in acute inflammatory response GO:0002541 ISO
    blood coagulation GO:0007596 ISO
    blood coagulation GO:0007596 TAS
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of platelet-derived growth factor receptor signaling pathway GO:0010641 ISO
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    protein processing GO:0016485 ISO
    cytokine-mediated signaling pathway GO:0019221 IBA
    positive regulation of TOR signaling GO:0032008 ISO
    positive regulation of interleukin-8 production GO:0032757 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 ISO
    membrane GO:0016020 IEA
    collagen-containing extracellular matrix GO:0062023 ISO
    serine-type peptidase complex GO:1905286 ISO
 Experiment description of studies that identified F3 in sEVs
1
Experiment ID 1101
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1102
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 1103
MISEV standards
EM
Biophysical techniques
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for F3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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