Gene description for Ext1
Gene name exostoses (multiple) 1
Gene symbol Ext1
Other names/aliases AA409028
Species Mus musculus
 Database cross references - Ext1
ExoCarta ExoCarta_14042
Vesiclepedia VP_14042
Entrez Gene 14042
UniProt P97464  
 Ext1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ext1
Molecular Function
    acetylglucosaminyltransferase activity GO:0008375 IBA
    glucuronosyltransferase activity GO:0015020 IBA
    glycosyltransferase activity GO:0016757 IMP
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity GO:0050508 IDA
    glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity GO:0050508 IGI
    glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity GO:0050508 ISO
    glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity GO:0050508 ISO
    N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity GO:0050509 IDA
    N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity GO:0050509 IGI
    N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity GO:0050509 ISO
Biological Process
    polysaccharide biosynthetic process GO:0000271 ISO
    cell morphogenesis GO:0000902 IMP
    ossification GO:0001503 ISO
    endochondral ossification GO:0001958 IMP
    blood vessel remodeling GO:0001974 IMP
    chondrocyte differentiation GO:0002062 IMP
    glandular epithelial cell differentiation GO:0002067 IMP
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    hypersensitivity GO:0002524 IMP
    heart field specification GO:0003128 IMP
    chondrocyte hypertrophy GO:0003415 IMP
    endochondral bone growth GO:0003416 IMP
    glycosaminoglycan biosynthetic process GO:0006024 ISO
    protein glycosylation GO:0006486 IEA
    vacuole organization GO:0007033 IMP
    cell adhesion GO:0007155 IMP
    cell surface receptor signaling pathway GO:0007166 IMP
    cell surface receptor signaling pathway GO:0007166 IMP
    smoothened signaling pathway GO:0007224 IMP
    gastrulation GO:0007369 IMP
    gastrulation GO:0007369 IMP
    axon guidance GO:0007411 IGI
    axon guidance GO:0007411 IMP
    axon guidance GO:0007411 IMP
    brain development GO:0007420 IMP
    brain development GO:0007420 IMP
    endoderm development GO:0007492 IMP
    endoderm development GO:0007492 IMP
    mesoderm development GO:0007498 IMP
    mesoderm development GO:0007498 IMP
    regulation of blood pressure GO:0008217 IGI
    cell population proliferation GO:0008283 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    response to virus GO:0009615 IMP
    response to light intensity GO:0009642 IMP
    gene expression GO:0010467 IMP
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IMP
    neural crest cell differentiation GO:0014033 IMP
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IDA
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IMP
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IMP
    heparan sulfate proteoglycan biosynthetic process GO:0015012 ISO
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 ISO
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 ISS
    cellular component organization GO:0016043 IMP
    stem cell division GO:0017145 IMP
    antigen processing and presentation GO:0019882 IMP
    optic nerve development GO:0021554 IMP
    olfactory bulb development GO:0021772 IMP
    hair cycle process GO:0022405 IMP
    protein catabolic process GO:0030163 IMP
    proteoglycan biosynthetic process GO:0030166 IMP
    collagen fibril organization GO:0030199 IMP
    heparin metabolic process GO:0030202 IMP
    chondroitin sulfate metabolic process GO:0030204 IMP
    heparin biosynthetic process GO:0030210 IGI
    heparin biosynthetic process GO:0030210 IMP
    heparin biosynthetic process GO:0030210 IMP
    BMP signaling pathway GO:0030509 IDA
    BMP signaling pathway GO:0030509 IMP
    hair follicle morphogenesis GO:0031069 IMP
    neuron projection development GO:0031175 IMP
    chemokine production GO:0032602 IMP
    glomerular basement membrane development GO:0032836 IMP
    cell adhesion mediated by integrin GO:0033627 IMP
    social behavior GO:0035176 IMP
    synaptic transmission, glutamatergic GO:0035249 IMP
    multicellular organism growth GO:0035264 IMP
    chondrocyte proliferation GO:0035988 IMP
    limb joint morphogenesis GO:0036022 IMP
    dendritic cell migration GO:0036336 IMP
    lymphocyte adhesion to endothelial cell of high endothelial venule GO:0036339 IMP
    fluid transport GO:0042044 IGI
    wound healing GO:0042060 IMP
    vasodilation GO:0042311 IGI
    fear response GO:0042596 IMP
    ossification involved in bone maturation GO:0043931 IMP
    cellular response to fibroblast growth factor stimulus GO:0044344 IMP
    cell fate commitment GO:0045165 IMP
    bone resorption GO:0045453 IMP
    embryonic morphogenesis GO:0048598 IMP
    sebaceous gland development GO:0048733 IMP
    multicellular organismal-level water homeostasis GO:0050891 IGI
    leukocyte tethering or rolling GO:0050901 IMP
    sulfation GO:0051923 IDA
    sulfation GO:0051923 IMP
    sodium ion homeostasis GO:0055078 IGI
    heart contraction GO:0060047 IGI
    canonical Wnt signaling pathway GO:0060070 IMP
    hematopoietic stem cell differentiation GO:0060218 IMP
    bone morphogenesis GO:0060349 IMP
    endochondral bone morphogenesis GO:0060350 IGI
    endochondral bone morphogenesis GO:0060350 IMP
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IMP
    epithelium development GO:0060429 IMP
    epithelial tube branching involved in lung morphogenesis GO:0060441 IMP
    mesenchyme development GO:0060485 IMP
    smoothened signaling pathway involved in lung development GO:0060506 IMP
    developmental growth involved in morphogenesis GO:0060560 IMP
    sweat gland development GO:0060792 IMP
    hematopoietic stem cell homeostasis GO:0061484 IMP
    motor behavior GO:0061744 IMP
    perichondral bone morphogenesis GO:0061974 IMP
    stomach development GO:0062094 IMP
    protein-containing complex assembly GO:0065003 IMP
    dendrite self-avoidance GO:0070593 IMP
    response to growth factor GO:0070848 IMP
    response to heparin GO:0071503 IMP
    vocalization behavior GO:0071625 IMP
    basement membrane organization GO:0071711 IMP
    cellular response to BMP stimulus GO:0071773 IMP
    podocyte differentiation GO:0072112 IMP
    embryonic skeletal joint development GO:0072498 IMP
    lymphocyte migration into lymphoid organs GO:0097021 IMP
    hematopoietic stem cell migration to bone marrow GO:0097241 IMP
    cellular response to virus GO:0098586 IMP
    bone growth GO:0098868 IMP
    tight junction organization GO:0120193 IMP
    mesenchymal cell differentiation involved in bone development GO:1901706 IMP
    cranial skeletal system development GO:1904888 IMP
    response to leukemia inhibitory factor GO:1990823 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISO
    synapse GO:0045202 IEA
    catalytic complex GO:1902494 ISO
    catalytic complex GO:1902494 ISS
 Experiment description of studies that identified Ext1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ext1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ext2  
Co-fractionation Mus musculus
2 Trap1 68015
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here