Gene description for Eno2
Gene name enolase 2, gamma neuronal
Gene symbol Eno2
Other names/aliases AI837106
D6Ertd375e
Eno-2
NSE
Species Mus musculus
 Database cross references - Eno2
ExoCarta ExoCarta_13807
Vesiclepedia VP_13807
Entrez Gene 13807
UniProt P17183  
 Eno2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Eno2
Molecular Function
    magnesium ion binding GO:0000287 IEA
    phosphopyruvate hydratase activity GO:0004634 IBA
    phosphopyruvate hydratase activity GO:0004634 IEA
    phosphopyruvate hydratase activity GO:0004634 ISO
    enzyme binding GO:0019899 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    gluconeogenesis GO:0006094 ISO
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    canonical glycolysis GO:0061621 ISO
Subcellular Localization
    phosphopyruvate hydratase complex GO:0000015 IBA
    phosphopyruvate hydratase complex GO:0000015 IEA
    phosphopyruvate hydratase complex GO:0000015 ISO
    photoreceptor inner segment GO:0001917 IDA
    intracellular anatomical structure GO:0005622 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 ISO
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    growth cone GO:0030426 ISO
    neuronal cell body GO:0043025 IDA
    perikaryon GO:0043204 IDA
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 ISO
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Eno2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Eno2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 MAPT  
Reconstituted Complex Homo sapiens
5 HSD17B10 3028
Affinity Capture-MS Homo sapiens
6 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Eno2 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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