Gene description for Dpep1
Gene name dipeptidase 1 (renal)
Gene symbol Dpep1
Other names/aliases AI327012
MBD
Species Mus musculus
 Database cross references - Dpep1
ExoCarta ExoCarta_13479
Vesiclepedia VP_13479
Entrez Gene 13479
UniProt P31428  
 Dpep1 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Dpep1
Molecular Function
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    beta-lactamase activity GO:0008800 IDA
    beta-lactamase activity GO:0008800 ISO
    dipeptidase activity GO:0016805 IDA
    dipeptidase activity GO:0016805 IMP
    dipeptidase activity GO:0016805 ISO
    GPI anchor binding GO:0034235 ISS
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISO
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 ISS
    metallodipeptidase activity GO:0070573 ISO
    metallodipeptidase activity GO:0070573 ISS
    modified amino acid binding GO:0072341 ISO
    modified amino acid binding GO:0072341 ISS
Biological Process
    proteolysis GO:0006508 IEA
    leukotriene metabolic process GO:0006691 IDA
    leukotriene metabolic process GO:0006691 IMP
    glutathione catabolic process GO:0006751 IMP
    inflammatory response GO:0006954 IMP
    antibiotic metabolic process GO:0016999 IDA
    antibiotic metabolic process GO:0016999 ISO
    antibiotic metabolic process GO:0016999 ISS
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of cell migration GO:0030336 ISS
    neutrophil chemotaxis GO:0030593 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    homocysteine metabolic process GO:0050667 ISO
    homocysteine metabolic process GO:0050667 ISS
    cellular response to calcium ion GO:0071277 ISS
    cellular response to xenobiotic stimulus GO:0071466 ISO
    cellular response to xenobiotic stimulus GO:0071466 ISS
    cellular response to nitric oxide GO:0071732 ISS
    leukotriene D4 catabolic process GO:1901749 ISO
    leukotriene D4 catabolic process GO:1901749 ISS
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISS
Subcellular Localization
    extracellular space GO:0005615 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    apical plasma membrane GO:0016324 IEA
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
    microvillus membrane GO:0031528 IEA
    apical part of cell GO:0045177 ISO
    apical part of cell GO:0045177 ISS
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified Dpep1 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Dpep1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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