Gene description for Dhcr7
Gene name 7-dehydrocholesterol reductase
Gene symbol Dhcr7
Other names/aliases AA409147
Species Mus musculus
 Database cross references - Dhcr7
ExoCarta ExoCarta_13360
Vesiclepedia VP_13360
Entrez Gene 13360
UniProt O88455  
 Dhcr7 identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Dhcr7
Molecular Function
    cholesterol-5,6-oxide hydrolase activity GO:0033963 ISS
    7-dehydrocholesterol reductase activity GO:0047598 IBA
    7-dehydrocholesterol reductase activity GO:0047598 IDA
    7-dehydrocholesterol reductase activity GO:0047598 ISO
    NADP binding GO:0050661 ISS
Biological Process
    blood vessel development GO:0001568 IMP
    cholesterol biosynthetic process GO:0006695 IBA
    cholesterol biosynthetic process GO:0006695 IEA
    cholesterol biosynthetic process GO:0006695 ISO
    post-embryonic development GO:0009791 IMP
    sterol biosynthetic process GO:0016126 IMP
    lung development GO:0030324 IMP
    multicellular organism growth GO:0035264 IMP
    regulation of cell population proliferation GO:0042127 IMP
    regulation of cholesterol biosynthetic process GO:0045540 ISO
    lung epithelial cell differentiation GO:0060487 IMP
Subcellular Localization
    nuclear outer membrane GO:0005640 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
 Experiment description of studies that identified Dhcr7 in sEVs
1
Experiment ID 264
MISEV standards
Biophysical techniques
CD63|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dhcr7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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