Gene description for Ddost
Gene name dolichyl-di-phosphooligosaccharide-protein glycotransferase
Gene symbol Ddost
Other names/aliases -
Species Mus musculus
 Database cross references - Ddost
ExoCarta ExoCarta_13200
Vesiclepedia VP_13200
Entrez Gene 13200
UniProt O54734  
 Ddost identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddost
Molecular Function
    enzyme activator activity GO:0008047 ISO
Biological Process
    protein glycosylation GO:0006486 ISS
    protein N-linked glycosylation GO:0006487 ISO
    protein N-linked glycosylation GO:0006487 NAS
    protein N-linked glycosylation via asparagine GO:0018279 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IEA
    protein N-linked glycosylation via asparagine GO:0018279 ISO
    regulation of protein stability GO:0031647 ISO
    response to cytokine GO:0034097 ISO
    T cell activation GO:0042110 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 NAS
    oligosaccharyltransferase complex GO:0008250 IBA
    oligosaccharyltransferase complex GO:0008250 ISO
    oligosaccharyltransferase complex GO:0008250 ISS
    oligosaccharyltransferase complex GO:0008250 NAS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Ddost in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddost
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Lnx2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ddost is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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