Gene description for Col4a2
Gene name collagen, type IV, alpha 2
Gene symbol Col4a2
Other names/aliases Col4a-2
Species Mus musculus
 Database cross references - Col4a2
ExoCarta ExoCarta_12827
Vesiclepedia VP_12827
Entrez Gene 12827
UniProt P08122  
 Col4a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col4a2
Molecular Function
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    molecular adaptor activity GO:0060090 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    DNA-templated transcription GO:0006351 IDA
    response to activity GO:0014823 IEA
    negative regulation of angiogenesis GO:0016525 ISO
    negative regulation of angiogenesis GO:0016525 ISS
    extracellular matrix organization GO:0030198 IBA
    endodermal cell differentiation GO:0035987 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IBA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IGI
    cellular response to transforming growth factor beta stimulus GO:0071560 IEP
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen type IV trimer GO:0005587 IBA
    collagen type IV trimer GO:0005587 IDA
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified Col4a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col4a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Col5a1 12831
Co-fractionation Mus musculus
3 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
4 Serpinh1 12406
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Mmp2  
Co-fractionation Mus musculus
7 Col1a2 12843
Co-fractionation Mus musculus
8 Col5a2  
Co-fractionation Mus musculus
9 Zfp106  
Co-fractionation Mus musculus
View the network image/svg+xml



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