Gene description for Cxcr4
Gene name chemokine (C-X-C motif) receptor 4
Gene symbol Cxcr4
Other names/aliases CD184
Cmkar4
LESTR
PB-CKR
PBSF/SDF-1
Sdf1r
b2b220Clo
Species Mus musculus
 Database cross references - Cxcr4
ExoCarta ExoCarta_12767
Vesiclepedia VP_12767
Entrez Gene 12767
UniProt P70658  
 Cxcr4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cxcr4
Molecular Function
    actin binding GO:0003779 ISO
    protein binding GO:0005515 IPI
    C-C chemokine receptor activity GO:0016493 IBA
    cytokine binding GO:0019955 IEA
    C-C chemokine binding GO:0019957 IBA
    C-C chemokine binding GO:0019957 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    myosin light chain binding GO:0032027 ISO
    small molecule binding GO:0036094 ISO
    C-X-C motif chemokine 12 receptor activity GO:0038147 ISO
    C-X-C motif chemokine 12 receptor activity GO:0038147 ISS
    ubiquitin binding GO:0043130 ISO
Biological Process
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    ameboidal-type cell migration GO:0001667 IMP
    neuron migration GO:0001764 IDA
    neuron migration GO:0001764 ISO
    kidney development GO:0001822 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    epithelial cell development GO:0002064 ISO
    ventricular septum development GO:0003281 IMP
    chemotaxis GO:0006935 IEA
    immune response GO:0006955 IBA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 IBA
    germ cell development GO:0007281 IMP
    nervous system development GO:0007399 TAS
    brain development GO:0007420 IBA
    brain development GO:0007420 IMP
    motor neuron axon guidance GO:0008045 IMP
    germ cell migration GO:0008354 IMP
    animal organ morphogenesis GO:0009887 TAS
    response to organic cyclic compound GO:0014070 ISO
    cell migration GO:0016477 ISO
    calcium-mediated signaling GO:0019722 IBA
    calcium-mediated signaling GO:0019722 ISO
    neurogenesis GO:0022008 IBA
    neurogenesis GO:0022008 ISO
    telencephalon cell migration GO:0022029 ISO
    regulation of cell adhesion GO:0030155 ISO
    regulation of cell migration GO:0030334 IGI
    regulation of cell migration GO:0030334 IMP
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of vascular wound healing GO:0035470 ISO
    hematopoietic stem cell migration GO:0035701 IMP
    aorta development GO:0035904 IMP
    CXCL12-activated CXCR4 signaling pathway GO:0038160 IDA
    CXCL12-activated CXCR4 signaling pathway GO:0038160 ISO
    CXCL12-activated CXCR4 signaling pathway GO:0038160 ISS
    T cell proliferation GO:0042098 IMP
    regulation of programmed cell death GO:0043067 ISO
    myelin maintenance GO:0043217 IMP
    blood vessel endothelial cell migration GO:0043534 IMP
    endothelial cell differentiation GO:0045446 ISO
    generation of neurons GO:0048699 IEP
    positive regulation of oligodendrocyte differentiation GO:0048714 IMP
    positive regulation of neurogenesis GO:0050769 ISO
    regulation of viral process GO:0050792 ISO
    regulation of chemotaxis GO:0050920 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    detection of temperature stimulus involved in sensory perception of pain GO:0050965 ISO
    detection of mechanical stimulus involved in sensory perception of pain GO:0050966 ISO
    regulation of calcium ion transport GO:0051924 ISO
    cardiac muscle contraction GO:0060048 ISO
    cell chemotaxis GO:0060326 IBA
    endothelial tube morphogenesis GO:0061154 ISO
    neural precursor cell proliferation GO:0061351 IEP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    cellular response to cytokine stimulus GO:0071345 IDA
    cellular response to cytokine stimulus GO:0071345 ISO
    cellular response to cytokine stimulus GO:0071345 ISS
    positive regulation of calcium ion import GO:0090280 IDA
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
    response to tacrolimus GO:1901327 ISO
    positive regulation of dendrite extension GO:1903861 ISO
    positive regulation of vasculature development GO:1904018 ISO
    positive regulation of mesenchymal stem cell migration GO:1905322 ISO
    positive regulation of macrophage migration inhibitory factor signaling pathway GO:2000448 ISO
    regulation of neuron migration GO:2001222 IMP
Subcellular Localization
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    endosome GO:0005768 ISO
    early endosome GO:0005769 ISO
    early endosome GO:0005769 ISO
    early endosome GO:0005769 ISS
    late endosome GO:0005770 ISO
    late endosome GO:0005770 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 IDA
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    growth cone GO:0030426 IDA
    cell leading edge GO:0031252 ISO
    cytoplasmic vesicle GO:0031410 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Cxcr4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cxcr4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itch 16396
Affinity Capture-Western Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Mtss1  
Affinity Capture-Western Mus musculus
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