Gene description for Cd9
Gene name CD9 antigen
Gene symbol Cd9
Other names/aliases Tspan29
Species Mus musculus
 Database cross references - Cd9
ExoCarta ExoCarta_12527
Vesiclepedia VP_12527
Entrez Gene 12527
UniProt P40240  
 Cd9 identified in sEVs derived from the following tissue/cell type
Adipocytes 36640325    
Adipocytes 36640325    
Amniotic fluid 17700640    
Brain 30967557    
Brain 30967557    
Brain 30967557    
Brain 30967557    
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Colorectal cancer cells 33431899    
Colorectal cancer cells 33431899    
Dendritic cells 10545503    
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Faeces 33431899    
Faeces 33431899    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney medullary cells 34580290    
Kidney medullary cells 34580290    
Kidney medullary cells 34580290    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 36648227    
Macrophages 36648227    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Mesenchymal stem cells 36253358    
Microglia 16081791    
Neural stem cells 25242146    
Neural stem cells 25242146    
Neural stem cells 25242146    
Plasma 33199621    
Plasma 33199621    
Plasma 32132711    
Plasma 32132711    
Plasma 32132711    
Podocytes 30967557    
Podocytes 30967557    
T-cell lymphoma cells 37309723    
Urine 17700640    
 Gene ontology annotations for Cd9
Molecular Function
    integrin binding GO:0005178 IPI
    integrin binding GO:0005178 ISO
    integrin binding GO:0005178 ISS
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 ISO
    cell adhesion GO:0007155 ISS
    single fertilization GO:0007338 IDA
    fusion of sperm to egg plasma membrane involved in single fertilization GO:0007342 IDA
    fusion of sperm to egg plasma membrane involved in single fertilization GO:0007342 ISO
    cell population proliferation GO:0008283 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    glial cell migration GO:0008347 IEA
    glial cell migration GO:0008347 ISO
    myoblast fusion involved in skeletal muscle regeneration GO:0014905 IMP
    paranodal junction assembly GO:0030913 IDA
    receptor internalization GO:0031623 IDA
    sperm-egg recognition GO:0035036 IDA
    cellular response to low-density lipoprotein particle stimulus GO:0071404 IDA
    negative regulation of platelet aggregation GO:0090331 ISO
    negative regulation of platelet aggregation GO:0090331 ISS
    regulation of macrophage migration GO:1905521 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    extracellular exosome GO:0070062 IDA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Cd9 in sEVs
1
Experiment ID 655
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36640325    
Organism Mus musculus
Experiment description Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis
Authors "Tang Y, Yang LJ, Liu H, Song YJ, Yang QQ, Liu Y, Qian SW, Tang QQ."
Journal name Cell Rep
Publication year 2023
Sample Adipocytes
Sample name Visceral (gonadal) adipose tissue
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
2
Experiment ID 656
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36640325    
Organism Mus musculus
Experiment description Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis
Authors "Tang Y, Yang LJ, Liu H, Song YJ, Yang QQ, Liu Y, Qian SW, Tang QQ."
Journal name Cell Rep
Publication year 2023
Sample Adipocytes
Sample name Visceral (gonadal) adipose tissue
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
3
Experiment ID 59
MISEV standards
✘ Biophysical techniques
✔
CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Mus musculus
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Amniotic fluid
Sample name Amniotic fluid
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1054
MISEV standards
✘ Biophysical techniques
✔
Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name WT mice - Fraction c
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 1055
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name WT mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1060
MISEV standards
✘ Biophysical techniques
✔
Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name KO mice - Fraction c
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1061
MISEV standards
✘ Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name KO mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
9
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 311
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 312
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
13
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
14
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
15
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
16
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
17
Experiment ID 1048
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 1049
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 1050
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 1051
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 1057
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name WT mice - Fraction c
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 1058
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name WT mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 1063
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name KO mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 1148
MISEV standards
✔
EM
Biophysical techniques
✔
Flot1|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
25
Experiment ID 1149
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
26
Experiment ID 1150
MISEV standards
✔
EM
Biophysical techniques
✔
Flot1|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
27
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 570
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 571
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 1104
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1105
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
37
Experiment ID 1106
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
38
Experiment ID 504
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63|Cd9
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36253358    
Organism Mus musculus
Experiment description Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM
Authors "Xu S, Cheuk YC, Jia Y, Chen T, Chen J, Luo Y, Cao Y, Guo J, Dong L, Zhang Y, Shi Y, Rong R."
Journal name Cell Death Dis
Publication year 2022
Sample Mesenchymal stem cells
Sample name Bone marrow-derived MSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
miRNA sequencing
qPCR
39
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
40
Experiment ID 262
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
41
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
42
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 315
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33199621    
Organism Mus musculus
Experiment description Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice
Authors "Castaño C, Mirasierra M, Vallejo M, Novials A, Párrizas M."
Journal name Proc Natl Acad Sci U S A
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RT-PCT
44
Experiment ID 316
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33199621    
Organism Mus musculus
Experiment description Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice
Authors "Castaño C, Mirasierra M, Vallejo M, Novials A, Párrizas M."
Journal name Proc Natl Acad Sci U S A
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RT-PCT
45
Experiment ID 333
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
46
Experiment ID 334
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
47
Experiment ID 335
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
48
Experiment ID 1052
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
49
Experiment ID 1053
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
50
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
51
Experiment ID 60
MISEV standards
✘ Biophysical techniques
✔
CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Mus musculus
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Cd9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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