Gene description for Cd63
Gene name CD63 antigen
Gene symbol Cd63
Other names/aliases C75951
ME491
Tspan30
Species Mus musculus
 Database cross references - Cd63
ExoCarta ExoCarta_12512
Vesiclepedia VP_12512
Entrez Gene 12512
UniProt P41731  
 Cd63 identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Brain 30967557    
Brain 30967557    
Brain 30967557    
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Breast cancer cells 30918259    
Colon cancer cells 37309723    
Colorectal cancer cells 33431899    
Colorectal cancer cells 33431899    
Embryonic fibroblasts 18494037    
Endothelial cells 34887361    
Endothelial cells 34887361    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Hepatocytes 19367702    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney 30967557    
Kidney 30967557    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 36648227    
Mast cells 17486113    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Melanoma cells 34593794    
Mesenchymal stem cells 36260670    
Mesenchymal stem cells 36253358    
Microglia 16081791    
Neural stem cells 25242146    
Neural stem cells 25242146    
Neural stem cells 25242146    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic duct epithelial cells 33827783    
Plasma 33199621    
Plasma 33199621    
Podocytes 30967557    
Podocytes 30967557    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd63
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 ISO
Biological Process
    positive regulation of receptor internalization GO:0002092 ISO
    positive regulation of receptor internalization GO:0002092 ISS
    cell-matrix adhesion GO:0007160 ISO
    cell-matrix adhesion GO:0007160 ISS
    protein localization GO:0008104 ISO
    negative regulation of epithelial cell migration GO:0010633 ISO
    protein transport GO:0015031 IEA
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    epithelial cell differentiation GO:0030855 ISO
    endosome to melanosome transport GO:0035646 ISO
    endosome to melanosome transport GO:0035646 ISS
    pigmentation GO:0043473 IMP
    positive regulation of cell adhesion GO:0045785 ISO
    positive regulation of endocytosis GO:0045807 ISO
    pigment granule maturation GO:0048757 ISO
    pigment granule maturation GO:0048757 ISS
    pigment cell differentiation GO:0050931 IMP
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 IBA
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 IMP
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 ISO
    regulation of potassium ion transmembrane transport GO:1901379 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 ISS
Subcellular Localization
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    late endosome GO:0005770 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell surface GO:0009986 ISO
    endosome membrane GO:0010008 ISO
    late endosome membrane GO:0031902 ISO
    late endosome membrane GO:0031902 ISS
    endosome lumen GO:0031904 IEA
    endosome lumen GO:0031904 ISO
    multivesicular body membrane GO:0032585 ISO
    multivesicular body membrane GO:0032585 ISS
    protein-containing complex GO:0032991 ISO
    melanosome GO:0042470 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    multivesicular body, internal vesicle GO:0097487 ISO
    multivesicular body, internal vesicle GO:0097487 ISS
 Experiment description of studies that identified Cd63 in sEVs
1
Experiment ID 89
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 1055
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name WT mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 1056
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name WT mice - Fraction e
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1061
MISEV standards
✘ Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Brain
Sample name KO mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 520
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name EO771
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 521
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name PyMT
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 944
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Mus musculus
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name 4T1-luci (+)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 311
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 312
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
12
Experiment ID 1089
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887361    
Organism Mus musculus
Experiment description Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Authors "Wang S, Shi M, Li J, Zhang Y, Wang W, Xu P, Li Y."
Journal name Aging
Publication year 2021
Sample Endothelial cells
Sample name Mouse aortic endothelial cells (MAEC)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 1090
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887361    
Organism Mus musculus
Experiment description Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Authors "Wang S, Shi M, Li J, Zhang Y, Wang W, Xu P, Li Y."
Journal name Aging
Publication year 2021
Sample Endothelial cells
Sample name Mouse aortic endothelial cells (MAEC)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Immunofluorescence
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
15
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Immunofluorescence
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
16
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 36
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Mus musculus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name MLP-29
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 695
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
19
Experiment ID 696
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
20
Experiment ID 697
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
21
Experiment ID 698
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - hepatocyte-specific Cas9 transgenic C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
qRT-PCR
22
Experiment ID 699
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - hepatocyte-specific Cas9 transgenic C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
qRT-PCR
23
Experiment ID 1048
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 1049
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 1050
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
26
Experiment ID 1051
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
27
Experiment ID 1057
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name WT mice - Fraction c
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 1058
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name WT mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 1059
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name WT mice - Fraction e
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 1062
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name KO mice - Fraction c
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 1063
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name KO mice - Fraction d
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1064
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Kidney
Sample name KO mice - Fraction e
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 552
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Mus musculus
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name LLC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
34
Experiment ID 553
MISEV standards
✘ Biophysical techniques
✔
Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Mus musculus
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name LLC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
35
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
36
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
37
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
38
Experiment ID 571
MISEV standards
✘ Biophysical techniques
✔
Hsp90|Gapdh|Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36648227    
Organism Mus musculus
Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
Authors "Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M."
Journal name mBio
Publication year 2023
Sample Macrophages
Sample name RAW264.2 - E. coli infected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
39
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
40
Experiment ID 1165
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
41
Experiment ID 1166
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
42
Experiment ID 1167
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
43
Experiment ID 1168
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
44
Experiment ID 1169
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
45
Experiment ID 1170
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
46
Experiment ID 1171
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
47
Experiment ID 1172
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name C57BL/6 bearing B16-F10 tumour
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
48
Experiment ID 1173
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
49
Experiment ID 1174
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
50
Experiment ID 1175
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
51
Experiment ID 1176
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
52
Experiment ID 1177
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
53
Experiment ID 1178
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
54
Experiment ID 1179
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
55
Experiment ID 1180
MISEV standards
✘ Biophysical techniques
✔
Cd63|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34593794    
Organism Mus musculus
Experiment description A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors "Wang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y."
Journal name Nat Commun
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Total Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
56
Experiment ID 499
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Immuno-EM
PubMed ID 36260670    
Organism Mus musculus
Experiment description "Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old mice"
Authors "Sanz-Ros J, Romero-García N, Mas-Bargues C, Monleón D, Gordevicius J, Brooke RT, Dromant M, Díaz A, Derevyanko A, Guío-Carrión A, Román-Domínguez A, Inglés M, Blasco MA, Horvath S, Viña J, Borrás C. "
Journal name Sci Adv
Publication year 2022
Sample Mesenchymal stem cells
Sample name Adipose MSCs from young C57BL/6J mice
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Flow cytometry
Immuno-EM
miRNA array
57
Experiment ID 499
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 36260670    
Organism Mus musculus
Experiment description "Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old mice"
Authors "Sanz-Ros J, Romero-García N, Mas-Bargues C, Monleón D, Gordevicius J, Brooke RT, Dromant M, Díaz A, Derevyanko A, Guío-Carrión A, Román-Domínguez A, Inglés M, Blasco MA, Horvath S, Viña J, Borrás C. "
Journal name Sci Adv
Publication year 2022
Sample Mesenchymal stem cells
Sample name Adipose MSCs from young C57BL/6J mice
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Flow cytometry
Immuno-EM
miRNA array
58
Experiment ID 504
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63|Cd9
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36253358    
Organism Mus musculus
Experiment description Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM
Authors "Xu S, Cheuk YC, Jia Y, Chen T, Chen J, Luo Y, Cao Y, Guo J, Dong L, Zhang Y, Shi Y, Rong R."
Journal name Cell Death Dis
Publication year 2022
Sample Mesenchymal stem cells
Sample name Bone marrow-derived MSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
miRNA sequencing
qPCR
59
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
60
Experiment ID 262
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
61
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
62
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
63
Experiment ID 1020
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
64
Experiment ID 1021
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
65
Experiment ID 1022
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
66
Experiment ID 1023
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
67
Experiment ID 1019
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
68
Experiment ID 315
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33199621    
Organism Mus musculus
Experiment description Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice
Authors "Castaño C, Mirasierra M, Vallejo M, Novials A, Párrizas M."
Journal name Proc Natl Acad Sci U S A
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RT-PCT
69
Experiment ID 316
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33199621    
Organism Mus musculus
Experiment description Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice
Authors "Castaño C, Mirasierra M, Vallejo M, Novials A, Párrizas M."
Journal name Proc Natl Acad Sci U S A
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RT-PCT
70
Experiment ID 1052
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
71
Experiment ID 1053
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
72
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd63
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc29a1 63959
Co-fractionation Mus musculus
2 Npc1 18145
Co-fractionation Mus musculus
3 Lnpep 240028
Co-fractionation Mus musculus
4 Eltd1  
Co-fractionation Mus musculus
5 Lamp2 16784
Co-fractionation Mus musculus
6 Cd47 16423
Co-fractionation Mus musculus
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