Gene description for Cd44
Gene name CD44 antigen
Gene symbol Cd44
Other names/aliases AU023126
AW121933
AW146109
HERMES
Ly-24
Pgp-1
Species Mus musculus
 Database cross references - Cd44
ExoCarta ExoCarta_12505
Vesiclepedia VP_12505
Entrez Gene 12505
UniProt P15379  
 Cd44 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic duct epithelial cells 33827783    
Pancreatic duct epithelial cells 33827783    
Pancreatic duct epithelial cells 33827783    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd44
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IBA
    cytokine receptor activity GO:0004896 IBA
    cytokine receptor activity GO:0004896 IDA
    cytokine receptor activity GO:0004896 ISO
    type II transforming growth factor beta receptor binding GO:0005114 IPI
    epidermal growth factor receptor binding GO:0005154 ISO
    protein binding GO:0005515 IPI
    hyaluronic acid binding GO:0005540 IBA
    hyaluronic acid binding GO:0005540 IMP
    hyaluronic acid binding GO:0005540 ISO
    channel regulator activity GO:0016247 ISO
    protein kinase binding GO:0019901 ISO
    cytokine binding GO:0019955 IDA
    cargo receptor activity GO:0038024 IDA
    cargo receptor activity GO:0038024 IMP
    cargo receptor activity GO:0038024 ISO
    phosphoprotein binding GO:0051219 ISO
Biological Process
    regulation of cell growth GO:0001558 ISO
    branching involved in ureteric bud morphogenesis GO:0001658 IMP
    wound healing involved in inflammatory response GO:0002246 IMP
    positive regulation of adaptive immune response GO:0002821 NAS
    negative regulation of mature B cell apoptotic process GO:0002906 IDA
    receptor-mediated endocytosis GO:0006898 IDA
    receptor-mediated endocytosis GO:0006898 IMP
    inflammatory response GO:0006954 IBA
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IEA
    cell adhesion GO:0007155 ISO
    positive regulation of gene expression GO:0010628 IGI
    Wnt signaling pathway GO:0016055 IDA
    cell migration GO:0016477 ISO
    cytokine-mediated signaling pathway GO:0019221 IEA
    hyaluronan catabolic process GO:0030214 IDA
    hyaluronan catabolic process GO:0030214 IMP
    hyaluronan catabolic process GO:0030214 ISO
    neuron projection development GO:0031175 ISO
    positive regulation of neutrophil apoptotic process GO:0033031 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    positive regulation of heterotypic cell-cell adhesion GO:0034116 ISO
    macrophage fusion GO:0034238 ISO
    T cell activation GO:0042110 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 IMP
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 ISO
    wound healing, spreading of cells GO:0044319 ISO
    wound healing, spreading of cells GO:0044319 ISS
    cellular response to fibroblast growth factor stimulus GO:0044344 ISO
    negative regulation of regulatory T cell differentiation GO:0045590 IMP
    negative regulation of inflammatory response GO:0050728 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    branching involved in prostate gland morphogenesis GO:0060442 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    monocyte aggregation GO:0070487 ISO
    positive regulation of monocyte aggregation GO:1900625 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 IMP
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    regulation of lamellipodium morphogenesis GO:2000392 ISO
    regulation of lamellipodium morphogenesis GO:2000392 ISS
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 IDA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    lamellipodium membrane GO:0031258 ISO
    lamellipodium membrane GO:0031258 ISS
    protein-containing complex GO:0032991 ISO
    macrophage migration inhibitory factor receptor complex GO:0035692 IBA
    macrophage migration inhibitory factor receptor complex GO:0035692 IDA
    macrophage migration inhibitory factor receptor complex GO:0035692 ISO
    cell projection GO:0042995 ISO
    cell projection GO:0042995 ISS
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Cd44 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 1020
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Immuno-EM
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
6
Experiment ID 1021
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Immuno-EM
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
7
Experiment ID 1034
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1035
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 1036
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 1037
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 1042
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 1043
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 1044
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 1045
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 1019
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 1046
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 1047
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd44
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
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