ExoCarta: Gene summary

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Gene description for Car6
Gene name	carbonic anhydrase 6
Gene symbol	Car6
Other names/aliases	Ca6 DOC1
Species	Mus musculus
Database cross references - Car6
ExoCarta	ExoCarta_12353
Vesiclepedia	VP_12353
Entrez Gene	12353
UniProt	P18761
Car6 identified in sEVs derived from the following tissue/cell type
Faeces	33431899
Faeces	33431899

Gene ontology annotations for Car6

Molecular Function
	carbonate dehydratase activity	GO:0004089	IBA
	carbonate dehydratase activity	GO:0004089	IEA
	zinc ion binding	GO:0008270	IEA
Biological Process
	detection of chemical stimulus involved in sensory perception of bitter taste	GO:0001580	IEA
	detection of chemical stimulus involved in sensory perception of bitter taste	GO:0001580	ISO
Subcellular Localization
	extracellular space	GO:0005615	IBA
	extracellular space	GO:0005615	IDA
	extracellular space	GO:0005615	ISO
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	IDA

Experiment description of studies that identified Car6 in sEVs

Experiment ID

303

MISEV standards
✔ EM	Biophysical techniques
✔ Cd63\|Cd81\|Cd9	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33431899

Organism

Mus musculus

Experiment description

High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance

Authors

"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."

Journal name

Nat Commun

Publication year

2021

Sample

Faeces

Sample name

Intestinal epithelial cells - C57BL/6

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
Lipid
miRNA

Methods used in the study

Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC