Gene description for Car4
Gene name carbonic anhydrase 4
Gene symbol Car4
Other names/aliases AW456718
Ca4
Species Mus musculus
 Database cross references - Car4
ExoCarta ExoCarta_12351
Vesiclepedia VP_12351
Entrez Gene 12351
UniProt Q64444  
 Car4 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Car4
Molecular Function
    carbonate dehydratase activity GO:0004089 IBA
    carbonate dehydratase activity GO:0004089 IDA
    carbonate dehydratase activity GO:0004089 ISO
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    regulation of pH GO:0006885 IC
    carbon dioxide transport GO:0015670 IC
    bicarbonate transport GO:0015701 IEA
    bicarbonate transport GO:0015701 ISO
Subcellular Localization
    rough endoplasmic reticulum GO:0005791 IEA
    rough endoplasmic reticulum GO:0005791 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 IEA
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 ISO
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    sarcoplasmic reticulum GO:0016529 ISO
    transport vesicle membrane GO:0030658 IEA
    transport vesicle membrane GO:0030658 ISO
    secretory granule membrane GO:0030667 IEA
    secretory granule membrane GO:0030667 ISO
    brush border membrane GO:0031526 IEA
    brush border membrane GO:0031526 ISO
    sarcolemma GO:0042383 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Car4 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Car4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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