Gene description for Bhmt
Gene name betaine-homocysteine methyltransferase
Gene symbol Bhmt
Other names/aliases -
Species Mus musculus
 Database cross references - Bhmt
ExoCarta ExoCarta_12116
Vesiclepedia VP_12116
Entrez Gene 12116
UniProt O35490  
 Bhmt identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Bhmt
Molecular Function
    methyltransferase activity GO:0008168 ISO
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    betaine-homocysteine S-methyltransferase activity GO:0047150 IBA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IDA
    betaine-homocysteine S-methyltransferase activity GO:0047150 ISO
Biological Process
    amino-acid betaine metabolic process GO:0006577 ISO
    amino-acid betaine catabolic process GO:0006579 IEA
    methionine biosynthetic process GO:0009086 IDA
    methionine biosynthetic process GO:0009086 ISO
    methylation GO:0032259 IEA
    'de novo' L-methionine biosynthetic process GO:0071266 ISO
    L-methionine salvage GO:0071267 IBA
    L-methionine salvage GO:0071267 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Bhmt in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Bhmt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
View the network image/svg+xml



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