Gene description for Slc7a1
Gene name solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Gene symbol Slc7a1
Other names/aliases 4831426K01Rik
AI447493
Atrc-1
Atrc1
Cat1
Rec-1
Rev-1
mCAT-1
Species Mus musculus
 Database cross references - Slc7a1
ExoCarta ExoCarta_11987
Vesiclepedia VP_11987
Entrez Gene 11987
UniProt Q09143  
 Slc7a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc7a1
Molecular Function
    L-ornithine transmembrane transporter activity GO:0000064 IBA
    L-ornithine transmembrane transporter activity GO:0000064 IDA
    virus receptor activity GO:0001618 IDA
    L-histidine transmembrane transporter activity GO:0005290 IEA
    L-histidine transmembrane transporter activity GO:0005290 ISO
    protein binding GO:0005515 IPI
    amino acid transmembrane transporter activity GO:0015171 ISO
    basic amino acid transmembrane transporter activity GO:0015174 ISO
    L-lysine transmembrane transporter activity GO:0015189 IBA
    L-lysine transmembrane transporter activity GO:0015189 IDA
    L-lysine transmembrane transporter activity GO:0015189 ISO
    L-arginine transmembrane transporter activity GO:0061459 IBA
    L-arginine transmembrane transporter activity GO:0061459 IDA
    L-arginine transmembrane transporter activity GO:0061459 ISO
Biological Process
    L-amino acid transport GO:0015807 ISO
    lysine transport GO:0015819 IEA
    lysine transport GO:0015819 ISO
    ornithine transport GO:0015822 ISO
    regulation of TOR signaling GO:0032006 ISO
    positive regulation of T cell proliferation GO:0042102 IEA
    positive regulation of T cell proliferation GO:0042102 ISO
    symbiont entry into host cell GO:0046718 IEA
    amino acid import across plasma membrane GO:0089718 ISO
    L-arginine import across plasma membrane GO:0097638 IBA
    L-arginine import across plasma membrane GO:0097638 ISO
    L-ornithine transmembrane transport GO:1903352 IBA
    L-ornithine transmembrane transport GO:1903352 IDA
    L-lysine transmembrane transport GO:1903401 IEA
    L-histidine import across plasma membrane GO:1903810 IEA
    L-histidine import across plasma membrane GO:1903810 ISO
    L-arginine transmembrane transport GO:1903826 IDA
    L-arginine transmembrane transport GO:1903826 IDA
    L-arginine transmembrane transport GO:1903826 ISO
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    basal plasma membrane GO:0009925 ISO
    membrane GO:0016020 IC
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Slc7a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc7a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
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