Gene description for Atp1b3
Gene name ATPase, Na+/K+ transporting, beta 3 polypeptide
Gene symbol Atp1b3
Other names/aliases AA409958
AI664000
AW212096
Species Mus musculus
 Database cross references - Atp1b3
ExoCarta ExoCarta_11933
Vesiclepedia VP_11933
Entrez Gene 11933
UniProt P97370  
 Atp1b3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp1b3
Molecular Function
    ATPase activator activity GO:0001671 IBA
    ATPase activator activity GO:0001671 IEA
    ATPase activator activity GO:0001671 ISO
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISO
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
Biological Process
    potassium ion transport GO:0006813 ISO
    sodium ion transport GO:0006814 ISO
    intracellular sodium ion homeostasis GO:0006883 IBA
    intracellular sodium ion homeostasis GO:0006883 IEA
    intracellular sodium ion homeostasis GO:0006883 ISO
    metal ion transport GO:0030001 ISO
    intracellular potassium ion homeostasis GO:0030007 IBA
    intracellular potassium ion homeostasis GO:0030007 IEA
    intracellular potassium ion homeostasis GO:0030007 ISO
    positive regulation of ATP-dependent activity GO:0032781 ISO
    sodium ion transmembrane transport GO:0035725 ISO
    sodium ion export across plasma membrane GO:0036376 IBA
    sodium ion export across plasma membrane GO:0036376 IEA
    sodium ion export across plasma membrane GO:0036376 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    potassium ion transmembrane transport GO:0071805 ISO
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    membrane repolarization GO:0086009 IEA
    membrane repolarization GO:0086009 ISO
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 ISO
    positive regulation of sodium ion export across plasma membrane GO:1903278 IEA
    positive regulation of sodium ion export across plasma membrane GO:1903278 ISO
    positive regulation of potassium ion import across plasma membrane GO:1903288 IEA
    positive regulation of potassium ion import across plasma membrane GO:1903288 ISO
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IEA
    potassium ion import across plasma membrane GO:1990573 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 ISO
    sodium:potassium-exchanging ATPase complex GO:0005890 IBA
    sodium:potassium-exchanging ATPase complex GO:0005890 IEA
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    caveola GO:0005901 ISO
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    sperm flagellum GO:0036126 IEA
    sperm flagellum GO:0036126 ISO
    melanosome GO:0042470 IEA
 Experiment description of studies that identified Atp1b3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp1b3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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