|
 Gene description for Serpinc1 |
| Gene name |
serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 |
| Gene symbol |
Serpinc1 |
| Other names/aliases |
AI114908
ATIII
At-3
At3 |
| Species |
Mus musculus |
| Database cross references - Serpinc1 |
| ExoCarta |
ExoCarta_11905 |
| Vesiclepedia |
VP_11905 |
| Entrez Gene |
11905 |
| UniProt |
P32261
|
| Serpinc1 identified in sEVs derived from the following tissue/cell type |
|
Colon cancer cells
|
37309723
|
|
Faeces
|
33431899
|
|
Faeces
|
33431899
|
|
Mast cells
|
17486113
|
|
T-cell lymphoma cells
|
37309723
|
| Gene ontology annotations for Serpinc1 |
|
|
| Experiment description of studies that identified Serpinc1 in sEVs |
| 1 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
303 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd63|Cd81|Cd9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33431899
|
| Organism |
Mus musculus |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Intestinal epithelial cells - C57BL/6 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC |
|
|
| 3 |
| Experiment ID |
304 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd63|Cd81|Cd9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33431899
|
| Organism |
Mus musculus |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Intestinal epithelial cells - C57BL/6 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC |
|
|
| 4 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 5 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for Serpinc1 |
|
|
| Pathways in which Serpinc1 is involved |
|
|
|
