Gene description for Ppat
Gene name phosphoribosyl pyrophosphate amidotransferase
Gene symbol Ppat
Other names/aliases Atase
Species Rattus norvegicus
 Database cross references - Ppat
ExoCarta ExoCarta_117544
Vesiclepedia VP_117544
Entrez Gene 117544
UniProt P35433  
 Ppat identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ppat
Molecular Function
    amidophosphoribosyltransferase activity GO:0004044 IBA
    amidophosphoribosyltransferase activity GO:0004044 IDA
    amidophosphoribosyltransferase activity GO:0004044 IEA
    amidophosphoribosyltransferase activity GO:0004044 ISO
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    iron-sulfur cluster binding GO:0051536 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IEP
    kidney development GO:0001822 IEP
    purine nucleotide biosynthetic process GO:0006164 IBA
    GMP biosynthetic process GO:0006177 IEA
    GMP biosynthetic process GO:0006177 ISO
    'de novo' IMP biosynthetic process GO:0006189 IEA
    'de novo' IMP biosynthetic process GO:0006189 ISO
    glutamine catabolic process GO:0006543 IDA
    lactation GO:0007595 IEP
    purine nucleobase biosynthetic process GO:0009113 IEA
    response to xenobiotic stimulus GO:0009410 IEP
    animal organ regeneration GO:0031100 IEP
    cellular response to insulin stimulus GO:0032869 IEP
    'de novo' AMP biosynthetic process GO:0044208 IEA
    'de novo' AMP biosynthetic process GO:0044208 ISO
    maternal process involved in female pregnancy GO:0060135 IEP
    cellular response to xenobiotic stimulus GO:0071466 IEP
    'de novo' XMP biosynthetic process GO:0097294 IEA
    'de novo' XMP biosynthetic process GO:0097294 ISO
 Experiment description of studies that identified Ppat in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here