Gene description for Rhoa
Gene name ras homolog family member A
Gene symbol Rhoa
Other names/aliases Arha
Arha2
Species Rattus norvegicus
 Database cross references - Rhoa
ExoCarta ExoCarta_117273
Entrez Gene 117273
UniProt P61589  
 Rhoa identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Rhoa
Molecular Function
    GTP binding GO:0005525 TAS
    myosin binding GO:0017022 ISO
    Rho GDP-dissociation inhibitor binding GO:0051022 IPI
    GDP binding GO:0019003 IDA
    GTPase activity GO:0003924 TAS
    protein domain specific binding GO:0019904 IPI
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell migration GO:0030335 IEP
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IMP
    stress fiber assembly GO:0043149 ISO
    response to mechanical stimulus GO:0009612 IEP
    cell-matrix adhesion GO:0007160 ISO
    cell adhesion GO:0007155 ISO
    regulation of dendrite development GO:0050773 IMP
    positive regulation of vasoconstriction GO:0045907 IEP
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 ISO
    response to glucose GO:0009749 IEP
    positive regulation of stress fiber assembly GO:0051496 ISO
    wound healing, spreading of cells GO:0044319 ISS
    negative chemotaxis GO:0050919 ISO
    positive regulation of neuron differentiation GO:0045666 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of lipase activity GO:0060193 ISO
    stress-activated protein kinase signaling cascade GO:0031098 IMP
    regulation of osteoblast proliferation GO:0033688 ISO
    negative regulation of cell death GO:0060548 IMP
    actin cytoskeleton organization GO:0030036 TAS
    androgen receptor signaling pathway GO:0030521 ISO
    cerebral cortex cell migration GO:0021795 ISO
    skeletal muscle tissue development GO:0007519 ISO
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
    regulation of calcium ion transport GO:0051924 IMP
    trabecula morphogenesis GO:0061383 ISO
    apical junction assembly GO:0043297 ISS
    positive regulation of actin filament polymerization GO:0030838 IMP
    ossification involved in bone maturation GO:0043931 ISO
    negative regulation of neuron differentiation GO:0045665 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    forebrain radial glial cell differentiation GO:0021861 ISO
    regulation of actin polymerization or depolymerization GO:0008064 IEP
    positive regulation of translation GO:0045727 IEP
    substantia nigra development GO:0021762 ISO
    response to amino acid GO:0043200 IEP
    regulation of cell migration GO:0030334 ISS
    positive regulation of cytokinesis GO:0032467 ISS
    mitotic spindle assembly GO:0090307 ISO
    apolipoprotein A-I-mediated signaling pathway GO:0038027 ISO
    response to hypoxia GO:0001666 IEP
    cytoskeleton organization GO:0007010 ISO
    GTP metabolic process GO:0046039 IDA
    cell differentiation GO:0030154 ISO
    cellular response to progesterone stimulus GO:0071393 NAS
    regulation of neural precursor cell proliferation GO:2000177 ISO
    response to ethanol GO:0045471 IEP
    neuron projection morphogenesis GO:0048812 IEP
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISS
    regulation of neuron projection development GO:0010975 ISO
    positive regulation of podosome assembly GO:0071803 ISO
    positive regulation of neuron apoptotic process GO:0043525 IMP
    cleavage furrow formation GO:0036089 ISS
    positive regulation of smooth muscle contraction GO:0045987 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    response to glucocorticoid GO:0051384 IEP
    response to drug GO:0042493 IEP
    positive regulation of cell adhesion GO:0045785 IEP
    cell morphogenesis GO:0000902 ISO
    positive regulation of cell growth GO:0030307 IMP
    skeletal muscle satellite cell migration GO:1902766 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoskeleton GO:0005856 IEA
    midbody GO:0030496 IEA
    focal adhesion GO:0005925 ISO
    lamellipodium GO:0030027 ISS
    vesicle GO:0031982 ISO
    ruffle membrane GO:0032587 ISO
    extracellular exosome GO:0070062 ISO
    apical junction complex GO:0043296 ISS
    cytosol GO:0005829 ISO
    axon GO:0030424 IDA
    cell cortex GO:0005938 ISS
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 ISO
    cleavage furrow GO:0032154 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Rhoa in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rhoa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rhoa is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here